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Streptavidin (HRP) is a Streptomyces avidinii Full Length protein, expressed in Native, with >95% purity and suitable for Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB.
Alternative names=Streptavidin
- Purity
>95% SDS-PAGE
- Expression system
Native
- Tags
Tag free
- Applications
Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB
- Biologically active
Yes
- Conjugation
HRP
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application IM | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/20000 - 1/200000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/10000 - 1/40000 | Notes - |
Images
Target data
Function
The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
Recommended products
Streptavidin (HRP) is a Streptomyces avidinii Full Length protein, expressed in Native, with >95% purity and suitable for Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB.
Alternative names=Streptavidin
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Native
- Tags
Tag free
- Applications
Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB
- Biologically active
Yes
- Biological activity
Binds to Biotin. Dissociation constant has not been measured.
- Conjugation
HRP
- Accession
- P22629-1
- Protein length
Full Length
- Animal free
No
- Nature
Native
- Species
Streptomyces avidinii
- Concentration
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: BSA, 0.87% Sodium chloride, 0.424% Tripotassium orthophosphate
Specifications
- Form
Liquid
- Additional notes
Streptavidin-HRP was prepared from chromatographically purified streptavidin.
General info
- Function
The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
- Sequence similarities
Belongs to the avidin/streptavidin family.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Horseradish peroxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.
This product has been assayed against 1.0 μg of Biotinylated IgG in a standard capture ELISA using the peroxidase substrate ABTS (ab142041) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
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