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Streptavidin (HRP) is a Streptomyces avidinii Full Length protein, expressed in Native, with >95% purity and suitable for Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (AB7403), expandable thumbnail
  • ELISA - Streptavidin (HRP) (AB7403), expandable thumbnail

Publications

  • Molecular neurobiology 61:5851-58672024
    The Role of PLAG1 in Mouse Brain Development and Neurogenesis.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Jemma G Gasperoni,Stephanie C Tran,Sylvia V H Grommen,Bert De Groef,Sebastian Dworkin
    PubMed 38240991
  • NPJ vaccines 8:1882023
    Subunit nanovaccine elicited T cell functional activation controls Trypanosoma cruzi mediated maternal and placental tissue damage and improves pregnancy outcomes in mice.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Lizette Elaine Rios,Nandadeva Lokugamage,Subhadip Choudhuri,Imran Hussain Chowdhury,Nisha Jain Garg
    PubMed 38104118

Key facts

Purity

>95% SDS-PAGE

Expression system

Native

Tags

Tag free

Applications

Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB

Biologically active

Yes

Conjugation

HRP

Reactivity data

Application

Dot

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IM

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ICC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/20000 - 1/200000

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

1/1000 - 1/5000

Notes

-

Application

IHC-Fr

Reactivity

Reacts

Dilution info

1/1000 - 1/5000

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

1/10000 - 1/40000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Alternative names

Recommended products

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Streptavidin (HRP) is a Streptomyces avidinii Full Length protein, expressed in Native, with >95% purity and suitable for Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB.

Alternative names

Key facts

Purity

>95% SDS-PAGE

Expression system

Native

Applications

Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB

Biological activity

Binds to Biotin. Dissociation constant has not been measured.

Conjugation

HRP

Accession
P22629-1
Animal free

No

Species

Streptomyces avidinii

Concentration
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Storage buffer

Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate

Sequence info

Amino acid sequence

Accession

P22629

Protein length

Full Length

Nature

Native

Specifications

Form

Liquid

Additional notes

Streptavidin-HRP was prepared from chromatographically purified streptavidin.

General info

Function

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Sequence similarities

Belongs to the avidin/streptavidin family.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Horseradish peroxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.

This product has been assayed against 1.0 μg of Biotinylated IgG in a standard capture ELISA using the peroxidase substrate ABTS (ABTS™, Peroxidase substrate ab142041) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (ab7403), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (ab7403)

    IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with Anti-Histone H1.0 antibody [27] ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (Goat Anti-Mouse IgG H&L (Biotin) ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • ELISA - Streptavidin (HRP) (ab7403), expandable thumbnail

    ELISA - Streptavidin (HRP) (ab7403)

    The wells were coated with Anti-Human IgG antibody [IG266] (Anti-Human IgG antibody [IG266] ab200699) at 1μg/ml at 50μl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG3 (ab138703) was then added starting at 20 μg/ml and plasma/serum at 1:500 and gradually diluted 1:4, 50μl/well for 2h. Biotin Anti-Human IgG3 antibody [EPR4419] ab201248 was then added at 1:10,000 dilution, 50μl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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