Streptavidin (HRP) is a Streptomyces avidinii Full Length protein, expressed in Native, with >95% purity and suitable for Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB.
>95% SDS-PAGE
Native
Tag free
Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB
Yes
HRP
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Application IM | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/20000 - 1/200000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/10000 - 1/40000 | Notes - |
Select an associated product type
The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
Streptavidin
Streptavidin (HRP) is a Streptomyces avidinii Full Length protein, expressed in Native, with >95% purity and suitable for Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB.
Streptavidin
>95% SDS-PAGE
Native
Tag free
Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB
Yes
Binds to Biotin. Dissociation constant has not been measured.
HRP
No
Streptomyces avidinii
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.42% Tripotassium orthophosphate
Liquid
Streptavidin-HRP was prepared from chromatographically purified streptavidin.
The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
Belongs to the avidin/streptavidin family.
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Horseradish peroxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.
This product has been assayed against 1.0 μg of Biotinylated IgG in a standard capture ELISA using the peroxidase substrate ABTS (ABTS™, Peroxidase substrate ab142041) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
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Full details and terms and conditions can be found here:
Terms & Conditions.
IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with Anti-Histone H1.0 antibody [27] ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (Goat Anti-Mouse IgG H&L (Biotin) ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
The wells were coated with Anti-Human IgG antibody [IG266] (Anti-Human IgG antibody [IG266] ab200699) at 1μg/ml at 50μl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG3 (ab138703) was then added starting at 20 μg/ml and plasma/serum at 1:500 and gradually diluted 1:4, 50μl/well for 2h. Biotin Anti-Human IgG3 antibody [EPR4419] ab201248 was then added at 1:10,000 dilution, 50μl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
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