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Streptavidin (HRP) is a Streptomyces avidinii Full Length protein, expressed in Native, with >95% purity and suitable for Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB.

Purity

>95% SDS-PAGE

Expression system

Native

Tags

Tag free

Applications

Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB

Biologically active

Yes

Conjugation

HRP

Reactivity data

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/20000 - 1/200000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/1000 - 1/5000
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
1/1000 - 1/5000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/10000 - 1/40000
Notes

-

Images

Target data

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Streptavidin (HRP) is a Streptomyces avidinii Full Length protein, expressed in Native, with >95% purity and suitable for Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB.

Key facts

Purity

>95% SDS-PAGE

Expression system

Native

Applications

Dot, IM, ICC, ELISA, IHC-P, IHC-Fr, WB

Biological activity

Binds to Biotin. Dissociation constant has not been measured.

Conjugation

HRP

Accession
P22629-1
Protein length

Full Length

Animal free

No

Nature

Native

Species

Streptomyces avidinii

Concentration
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Storage buffer

Preservative: 0.01% Gentamicin sulphate
Constituents: BSA, 0.87% Sodium chloride, 0.424% Tripotassium orthophosphate

Specifications

Form

Liquid

Additional notes

Streptavidin-HRP was prepared from chromatographically purified streptavidin.

General info

Function

The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Sequence similarities

Belongs to the avidin/streptavidin family.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Horseradish peroxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.

This product has been assayed against 1.0 μg of Biotinylated IgG in a standard capture ELISA using the peroxidase substrate ABTS (ab142041) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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