Streptavidin HRP (ready-to-use) is a Streptomyces avidinii protein and suitable for IHC-P.
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- Current stem cell research & therapy 19:725-7342024Use of Mesenchymal Stem Cells in Experimental Ovarian Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanan Fouad,Ibrahim A Albahlol,Hazim A Wahab,Eman H Nadwa,Heba M Galal,Mohamed Abouelkheir,Ahmed E Taha,Abdelkarim G Kamel,Hassan A AbdelmawllaPubMed 37448361
- Nutrients 15:2023Effect of Oleoylethanolamide-Based Dietary Supplement on Systemic Inflammation in the Development of Alimentary-Induced Obesity in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDarya Ivashkevich,Arina Ponomarenko,Igor Manzhulo,Ruslan Sultanov,Inessa DyuizenPubMed 37892420
- In vivo (Athens, Greece) 37:1562-15752023Role of Reduced Bdnf Expression in Novel Apc Mutant Allele-induced Intestinal and Colonic Tumorigenesis in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyşenur Gok,Aynur Işik,Sinem Bakir,Sarp Uzun,Gunes Guner,Ozge Ozcan,Baris Cerci,Ilyas Onbaşilar,Aytekin AkyolPubMed 37369509
- Scientific reports 13:99232023HOXA3 accelerates wound healing in diabetic and aged non-diabetic mammals.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK Parella,K Moody,D Wortel,H Colegrove,J A ElserPubMed 37337031
- Frontiers in pharmacology 14:11183582023Empagliflozin increases kidney weight due to increased cell size in the proximal tubule S3 segment and the collecting duct.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrederick Sinha,Anna Federlein,Annika Biesold,Magdalena Schwarzfischer,Katharina Krieger,Frank Schweda,Philipp TauberPubMed 37033639
- Cellular and molecular life sciences : CMLS 79:5602022Characterization of microtubule-associated protein tau isoforms and Alzheimer's disease-like pathology in normal sheep (Ovis aries): relevance to their potential as a model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmma S Davies,Russell M Morphew,David Cutress,A Jennifer Morton,Sebastian McBridePubMed 36269420
- International journal of molecular sciences 23:2022Evaluating Thera-101 as a Low-Volume Resuscitation Fluid in a Model of Polytrauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica Stukel Shah,Joseph Macaitis,Bridney Lundquist,Brian Johnstone,Michael Coleman,Michelle A Jefferson,Jacob Glaser,Annette R Rodriguez,Sylvain Cardin,Heuy-Ching Wang,Alexander BurdettePubMed 36293520
- Frontiers in genetics 13:9334162022The interconnected relationships between middle ear bulla size, cavitation defects, and chronic otitis media revealed in a syndromic mouse model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan M Fons,Natalie J Milmoe,Michael R G Dack,Leena Joshi,Hannah Thompson,Abigail S TuckerPubMed 36299576
- Biomaterials advances 139:2130352022The generation of a lactate-rich environment stimulates cell cycle progression and modulates gene expression on neonatal and hiPSC-derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJesús Ordoño,Soledad Pérez-Amodio,Kristen Ball,Aitor Aguirre,Elisabeth EngelPubMed 35907761
- Cancers 14:2022Inhibition of a Mitochondrial Potassium Channel in Combination with Gemcitabine and Abraxane Drastically Reduces Pancreatic Ductal Adenocarcinoma in an Immunocompetent Orthotopic Murine Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiwei Li,Gregory C Wilson,Magdalena Bachmann,Jiang Wang,Andrea Mattarei,Cristina Paradisi,Michael J Edwards,Ildiko Szabo,Erich Gulbins,Syed A Ahmad,Sameer H PatelPubMed 35681598
Key facts
- Applications
- IHC-P
- Biologically active
- No
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
Alternative names
Streptavidin
Recommended products
Streptavidin HRP (ready-to-use) is a Streptomyces avidinii protein and suitable for IHC-P.
Key facts
- Applications
- IHC-P
- Biologically active
- No
- Accession
- P22629-1
- Animal free
- No
- Species
- Streptomyces avidinii
- Concentration
- Storage buffer
pH: 7.6
Preservative: 0.2% Proclin 300
Constituents: PBS
Specifications
- Form
- Liquid
General info
- Function
The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
- Sequence similarities
Belongs to the avidin/streptavidin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Streptavidin-HRP for use with biotinylated secondary antibodies during IHC / immunohistochemistry. Optimized, ready-to-use format.
This product was previously called Streptavidin-Peroxidase.
IHC protocol suitable for use with Streptavidin-HRP ab64269:
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in antibody diluent and incubate.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP ab64269 and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved. Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin HRP (ready-to-use) (ab64269)
ab64269 Straptavidin Peroxidase used along with an anti-CD20 antibody on Human Tonsil
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin HRP (ready-to-use) (ab64269)
Human colon carcinoma stained with anti-p53 antibody using Streptavidin Peroxidase (ab64269).
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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