Streptavidin-Phycoerythrin (R-PE) is a Streptomyces avidinii Full Length protein, expressed in Escherichia coli and suitable for Flow Cyt.
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Key facts
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- Flow Cyt
- Biologically active
- No
- Conjugation
- PE
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
Alternative names
Streptavidin
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Streptavidin-Phycoerythrin (R-PE) is a Streptomyces avidinii Full Length protein, expressed in Escherichia coli and suitable for Flow Cyt.
Key facts
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- Flow Cyt
- Biologically active
- No
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Accession
- P22629-1
- Animal free
- No
- Species
- Streptomyces avidinii
- Concentration
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 2.9% Sodium chloride, 0.12% Sodium dihydrogen phosphate
Specifications
- Form
- Liquid
General info
- Function
The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
- Sequence similarities
Belongs to the avidin/streptavidin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store in the dark
Notes
Streptavidin-PE will bind to biotin allowing detection of biotinylated antibodies in Flow, microplate assays and microarrays. Streptavidin high affinity for biotin allows low background in cell sorting, immunofluorescence, in situ hybridization, or flow cytometry. Suitable for use with Mouse, Human and Rat samples.
Excitation: Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm)
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1 product image
Flow Cytometry - Streptavidin-Phycoerythrin (R-PE) (ab239759)
C57Bl/6 splenocytes were stained with Biotin Anti-Mouse CD19 (solid line) or Biotin Rat IgG2a isotype control (dashed line), followed by 0.06 μg ab239759.
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