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AB326318

Sudan virus-like particles (SUDV VLP)

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Sudan virus-like particles (SUDV VLP) is a Sudan ebolavirus - Uganda (2000) protein, expressed in Sf9 cells, with >75%, 2.662 EU/mg endotoxin level, suitable for WB.

View Alternative Names

Matrix protein VP40, Ebola VP40, Membrane-associated protein VP40, eVP40

1 Images
Western blot - Sudan virus-like particles (SUDV VLP) (AB326318)
  • WB

Supplier Data

Western blot - Sudan virus-like particles (SUDV VLP) (AB326318)

Western Blot was used to confirm the presence of Sudan-specific GP, NP and VP40 in the VLP. VLP sample was heat-denatured under reducing condition and loaded at 500 ng (A) and 100 ng (B & C).

(A) GP was detected as a broadly-diffused band ~37160 kDa using 0.1 µg/mL of Anti-SUDV GP mouse mAb (2H5)

(B) NP was detected as a prominent band between 80 and 110 kDa using 0.1 µg/mL of Anti-SUDV NP rabbit pAb

(C) VP40 was detected as multiple protein bands with one duplet band at ~40 kDa using 0.1 µg/mL of Anti-SUDV VP40 rabbit pAb.

Lane 1:

Western blot - Sudan virus-like particles (SUDV VLP) (ab326318) at 500 ng

Lanes 2 - 3:

Western blot - Sudan virus-like particles (SUDV VLP) (ab326318) at 100 ng

Observed band size: 37160 kDa,80 kDa,110 kDa,40 kDa

false

Key facts

Purity

>75% SDS-PAGE

Endotoxin level

2.662 EU/mg

Expression system

Sf9 cells

Tags

Tag free

Applications

WB

applications

Biologically active

No

Accession

Q5XX06

Animal free

No

Carrier free

No

Species

Sudan ebolavirus - Uganda (2000)

Storage buffer

Constituents: PBS, 10% Glycerol (glycerin, glycerine), 0.9% L-Arginine, 0.7% Glutamic Acid

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"100-500 ng", "notes":"<p></p>" } } }
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Product details

Sudan Virus-like particles (VLP’s) expressing recombinant Sudan virus (SUDV) glycoprotein (GP), nucleoprotein (NP), and matrix protein (VP40). These VLP’s are produced in Sf9 insect cells through infection with a recombinant baculovirus. Since these VLP’s mimic Sudan virus but do not contain genetic material, thus are not infectious and make an ideal candidate as a vaccine and also as a tool to enhance filovirus research.
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Sequence info

[{"linker":null,"sequence":"","proteinLength":null,"predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Sf9 cells","accessionNumber":"Q5XX06","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage duration
A few weeks
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis).

Sequence similarities

Belongs to the filoviridae matrix protein VP40 family.

Subcellular localisation

Host late endosome membrane

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Product protocols

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Target data

Plays an essential role virus particle assembly and budding. Acts by interacting with viral ribonucleocapsid and host members of the ESCRT (endosomal sorting complex required for transport) system such as host VPS4, PDCD6IP/ALIX, NEDD4 or TGS101. The interaction with host E3 ubiquitin ligase SMURF2 also facilitates virus budding. May play a role in immune cell dysfunction by being packaged into exosomes that can decrease the viability of recipient cells (via RNAi suppression and exosome-bystander apoptosis).
See full target information VP40
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Product promise

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