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AB307137

Anti-TMEM175 antibody [EPR24415-47] - Blocking Peptide

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Anti-TMEM175 antibody [EPR24415-47] - Blocking Peptide is a Synthetic blocking peptide.

View Alternative Names

Endosomal/lysosomal proton channel TMEM175, Potassium channel TMEM175, Transmembrane protein 175, hTMEM175, TMEM175

Key facts

Purity

undefined HPLC

Tags

Tag free

Applications

BL

applications

Biologically active

No

Accession

Q9BSA9

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

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Complementary Products

Reagents, Controls & Accessories

AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

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Primary Antibodies

AB300457

Anti-TMEM175 antibody [EPR24415-47]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM175 antibody [EPR24415-47] (AB300457)

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Cell Lines & Lysates

AB265571

Human TMEM175 knockout HeLa cell line

Western blot - Human TMEM175 knockout HeLa cell line (AB265571)

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Cell Lines & Lysates

AB258722

Human TMEM175 knockout HeLa cell lysate

Western blot - Human TMEM175 knockout HeLa cell lysate (AB258722)

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Assay Kits

AB309570

Human TMEM175 Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human TMEM175 Antibody Pair - BSA and Azide free (AB309570)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":null,"proteinLength":null,"predictedMolecularWeight":"55 kDa","actualMolecularWeight":"45 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Synthetic","expressionSystem":null,"accessionNumber":"Q9BSA9","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
-20°C
False
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Specifications

Form

Lyophilized

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General info

Function

Proton-activated proton channel that catalyzes proton efflux from endosomes and lysosomes to maintain a steady-state pH (PubMed : 35333573, PubMed : 35750034, PubMed : 37390818). Activated at low pH (under pH 4.6) by luminal side protons : selectively mediates lysosomal proton release from lysosomes, eliciting a proton leak that balances V-ATPase activity to maintain pH homeostasis (PubMed : 35750034). Regulation of lumenal pH stability is required for autophagosome-lysosome fusion (PubMed : 26317472, PubMed : 32267231). Also acts as a potassium channel at higher pH, regulating potassium conductance in endosomes and lysosomes (PubMed : 26317472, PubMed : 28723891, PubMed : 32228865, PubMed : 32267231, PubMed : 33505021). Constitutes the pore-forming subunit of the lysoK(GF) complex, a complex activated by extracellular growth factors (PubMed : 33505021). The lysoK(GF) complex is composed of TMEM175 and AKT (AKT1, AKT2 or AKT3), a major target of growth factor receptors : in the complex, TMEM175 channel is opened by conformational changes by AKT, leading to its activation (PubMed : 33505021). The lysoK(GF) complex is required to protect neurons against stress-induced damage (PubMed : 33505021).

Sequence similarities

Belongs to the TMEM175 family.

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Product protocols

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Target data

Proton-activated proton channel that catalyzes proton efflux from endosomes and lysosomes to maintain a steady-state pH (PubMed : 35333573, PubMed : 35750034, PubMed : 37390818). Activated at low pH (under pH 4.6) by luminal side protons : selectively mediates lysosomal proton release from lysosomes, eliciting a proton leak that balances V-ATPase activity to maintain pH homeostasis (PubMed : 35750034). Regulation of lumenal pH stability is required for autophagosome-lysosome fusion (PubMed : 26317472, PubMed : 32267231). Also acts as a potassium channel at higher pH, regulating potassium conductance in endosomes and lysosomes (PubMed : 26317472, PubMed : 28723891, PubMed : 32228865, PubMed : 32267231, PubMed : 33505021). Constitutes the pore-forming subunit of the lysoK(GF) complex, a complex activated by extracellular growth factors (PubMed : 33505021). The lysoK(GF) complex is composed of TMEM175 and AKT (AKT1, AKT2 or AKT3), a major target of growth factor receptors : in the complex, TMEM175 channel is opened by conformational changes by AKT, leading to its activation (PubMed : 33505021). The lysoK(GF) complex is required to protect neurons against stress-induced damage (PubMed : 33505021).
See full target information TMEM175
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Product promise

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