JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB241023

Albumin Depletion Kit

Be the first to review this product! Submit a review

|

(2 Publications)

Albumin Depletion Kit ab241023 provides easy and user-friendly spin columns for the depletion/removal of abundant albumin protein from samples for downstream applications.

- Uses Cibacron blue immobilized on beads for albumin binding
- Each spin column can bind ~2-3 mg albumin
- Produce ready-to- use albumin-depleted samples in 60 minutes
1 Images
Functional Studies - Albumin Depletion Kit (AB241023)
  • FuncS

Supplier Data

Functional Studies - Albumin Depletion Kit (AB241023)

Samples were analyzed by 15% SDS-PAGE. M : Protein Marker, 1 : Eluted albumin fraction (20 μL of concentrated eluted albumin, primarily containing albumin), 2 : Albumin-depleted plasma sample (20 μL of combined and concentrated flow-through and wash fractions).

Key facts

Sample types

Purified protein

style
left-column

Product details

Albumin Depletion Kit (ab241023) provides easy and user-friendly spin columns for the depletion/removal of abundant albumin protein from samples thus enhancing the sample analysis. The kit is based on the interaction of Cibacron Blue 3G-A (immobilized on beads) with albumin. Each spin column can bind ~2-3 mg of albumin and processes samples in less than one hour. After albumin depletion, samples can be used for subsequent ELISA, immunoassays and other downstream analyses such as gel electrophoresis and functional assays. These columns can also be used for purification and enrichment of a variety of Cibacron Blue dye-binding proteins.

Please note, this kit has only been tested on human samples.

This product is manufactured by BioVision, an Abcam company and was previously called K6573 EZAlbumin™ Depletion Kit (Spin Column). K6573-25 is the same size as the 25 test size of ab241023.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style
left-column

What's included?

{ "values": { "25Test": { "sellingSize": "25 Test", "publicAssetCode":"ab241023-25Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Cibacron Elution Buffer", "number":"AB241023-CMP03" }, { "size":"1 x 25 Vial", "name":"Cibacron Blue Column", "number":"AB241023-CMP02" }, { "size":"1 x 33 mL", "name":"Albumin Binding Buffer", "number":"AB241023-CMP01" } ] }, "25Test": { "sellingSize": "25 Test", "publicAssetCode":"ab241023-25Test", "assetComponentDetails": [ { "size":"25 x 1 Unit", "name":"Cibacron Blue Column", "number":"AB241023-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"Cibacron Elution Buffer", "number":"AB241023-CMP03", "productcode":"" }, { "size":"1 x 33 mL", "name":"Albumin Binding Buffer", "number":"AB241023-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
style
left-column

Product protocols

style
left-column

Target data

style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:9104 PubMed38643249

2024

SMS121, a new inhibitor of CD36, impairs fatty acid uptake and viability of acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Åbacka,Samuele Masoni,Giulio Poli,Peng Huang,Francesco Gusso,Carlotta Granchi,Filippo Minutolo,Tiziano Tuccinardi,Anna K Hagström-Andersson,Karin Lindkvist-Petersson

Nature protocols 18:1462-1487 PubMed36914899

2023

Comprehensive isolation of extracellular vesicles and nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Zhang,Dennis K Jeppesen,James N Higginbotham,Jeffrey L Franklin,Robert J Coffey
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB179887

Human Albumin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Albumin ELISA Kit (AB179887)

  • 3
  • 1
Assay Kits

AB65339

Creatine Assay Kit (Colorimetric/Fluorometric)

Functional Studies - Creatine Assay Kit (Colorimetric/Fluorometric) (AB65339)

  • 0
  • 0
Assay Kits

AB241035

ALT Assay Kit (384 well, Colorimetric/Fluorometric)

Functional Studies - ALT Assay Kit (384 well, Colorimetric/Fluorometric) (AB241035)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com