Skip to main content

Antibody Purification Kit - Nanoparticles (ab204909) removes common buffer constituents found in commercial antibodies.

This product has no reviews yet! Submit a review

What's included?

3 Test
Components
Additional Collecting Tubes
12 x 1 Vial
Binding Buffer (10X)
1 x 250 µL
Elution Buffer
1 x 1500 µL
Neutralizer
1 x 250 µL
Protein A resin
3 x 100 µL
Spin cartridge/ collecting tube assemblies
3 x 1 Unit
Wash Buffer
1 x 12 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Antibody Purification Kit - Nanoparticles (ab204909) removes common buffer constituents found in commercial antibodies.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Antibody Purification Kit - Nanoparticles (ab204909) removes common buffer constituents found in commercial antibodies (e.g. BSA, glycine, tris and azide) that decrease the efficiency of conjugation reactions with nanoparticles using our nanoparticle conjugation kits. ab204909 can also be used to purify antibodies from crude samples such as ascites fluid or serum. The antibody should ideally be in a volume of 100 - 500 μL. Up to 500 μg of antibody can be purified in each run.

The method involves capture of the antibody on Protein A Resin and removal of unwanted substances by a simple wash procedure, using a standard microfuge. The purified antibody is then eluted and neutralized.

Antibodies purified using the Antibody Purification Kit - Nanoparticles are fully compatible with our nanoparticle conjugation kits (Gold, Latex, Europium and Magnetic). To purify antibodies for use with our other antibody conjugation kits, we recommend using one of our other purification kits - view our selection guide.

For purifying mouse IgG, we recommend using Mouse Antibody Purification Kit - Nanoparticles ab204910.

This product is manufactured by Expedeon, an Abcam company, and has previously been called AbPure™ Protein A Purification System and GOLD antibody purification kit. 260-0005 is the same as the 1 test size. 260-0010 is the same as the 3 tests size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com