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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants.

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What's included?

3 Test
Components
Additional Collecting Tubes
12 x 1 Vial
Binding Buffer (10X)
1 x 200 µL
Elution Buffer
1 x 1500 µL
Neutralizer Buffer
1 x 500 µL
Protein A resin
3 x 100 µL
Spin cartridge/ collecting tube assemblies
3 x 1 Unit
Wash Buffer
1 x 12 mL

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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up is ideally in a volume of 100-500 μl.

Up to 20-500 μg of antibody can be purified in each run.

The method involves capture of the antibody on Protein A resin and the removal of unwanted substances by a simple wash procedure, which is carried out in a standard microfuge. The purified product is then eluted and neutralized.

Note: This method cannot be used with samples containing relatively dilute antibody in large volumes (e.g. tissue culture supernatant). For larger volumes, we recommend the use of Antibody TCS Purification Kit (3 purifications) (ab109207) or Antibody Serum Purification kit (3 purifications) (ab109209).

Antibodies purified using the Antibody Purification Kit (Protein A) are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our GOLD Antibody conjugation kits. To purify antibodies for use with our Gold conjugation kits, please use our Gold antibody purification kit (ab204909)

ab102784 is not suitable for goat antibody purification. Please refer to the protocol booklet for more information about suitable and unsuitable species antibodies."

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ Antibody Purification System. 860-0005 is the same as the 1 test size. 860-0010 is the same as the 3 tests size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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