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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128747 quickly removes these contaminants.

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What's included?

3 Test
Components
Additional Collecting Tubes
12 x 1 Vial
Elution Buffer
1 x 1500 µL
G Binding Buffer (10X)
1 x 200 µL
Neutralizer Buffer
1 x 500 µL
Protein G Resin
3 x 400 µL
Spin cartridge/ collecting tube assemblies
3 x 1 Unit
Wash Buffer
1 x 12 mL

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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128747 quickly removes these contaminants.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128747 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up should be in a volume of 100 to 500 μL. 20 to 300 μg of antibody can be purified in each run.

The method involves capturing the antibody on the Protein G resin. Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. Once the antibody has bound to the Protein G, unwanted substances can be removed by simply washing the resin. Up to 300μg of antibody can be purified in each run. The purified product is then eluted and neutralized.

Antibodies purified with the Antibody Purification Kit (Protein G) are fully compatible with our Lightning-Link® Antibody Conjugation kits and Oligonucleotide Conjugation Kit. To purify antibodies for use with our Gold conjugation kits, please use our Gold antibody purification kit (ab204909).

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ G Antibody Purification System. 890-0005 is the same as the 1 test size. 890-0010 is the same as the 3 tests size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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