Antibody Purification Kit (Protein G)

Product details

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. Antibody Purification Kit (Protein G)(ab128747 quickly) removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up should be in a volume of 100 to 500 µL.

20 to 300 µg of antibody can be purified in each run.

The method involves capturing the antibody on the Protein G resin. Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. Once the antibody has bound to the Protein G, unwanted substances can be removed by simply washing the resin. The purified product is then eluted and neutralized.

Antibodies purified with the Antibody Purification Kit (Protein G) are fully compatible with our Lightning-Link^® Antibody Conjugation kits and Oligonucleotide Conjugation Kit. To purify antibodies for use with our Gold conjugation kits, please use our Gold antibody purification kit (ab204909).

Abcam's antibody purification and concentration kitsoffer you a streamlined and efficient solution for isolating and concentrating antibodies from various sources. These kits are designed with simplicity and effectiveness in mind, allowing you to achieve high-purity antibodies with minimal hands-on time. Using our advanced purification technologies, we ensure that your antibodies retain their functionality and specificity, making them ideal for a wide range of applications, including western blot, immunohistochemistry (IHC), and ELISA.

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Learn about our Lightning-Link® antibody and Oligonucleotide labeling kits and their advantages here.