Lentivirus and Retrovirus Purification Kit ab288104 (previously known as Mag-Lentivirus and Retrovirus Purification Kit K1458) uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation.
Images
Key facts
- Sample type
- Serum, Cell Lysate
What's included?
Recommended products
Lentivirus and Retrovirus Purification Kit ab288104 (previously known as Mag-Lentivirus and Retrovirus Purification Kit K1458) uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation.
Key facts
- Sample type
- Serum, Cell Lysate
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Lentivirus and Retrovirus Purification Kit ab288104 (previously known as Mag-Lentivirus and Retrovirus Purification Kit K1458) uses magnetic nanoparticles to capture viruses by electrostatic and hydrophobic interactions with 80-99% efficiency and the purification process does not need ultracentrifugation. The viruses captured on Lentivirus (LV)-Retrovirus (RV)-magnetic beads can be used directly for multiple assays. Alternatively, viruses can be eluted with the elution buffer and used for downstream applications.
This product is manufactured by BioVision, an Abcam company and was previously called K1458 Lentivirus and Retrovirus Purification Kit. K1458-20 is the same size as the 20 test size of ab288104.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Functional Studies - Lentivirus and Retrovirus Purification Kit (ab288104)
Lentivirus and Retrovirus Purification Kit.
Efficiency of viral capture. Virus solution containing 107 viral particles/ml of HIV-SFFV-GFP was mixed with LV/RV-magnetic beads and captured by the magnetic field. Both the supernatant (SN) and re-suspended magnetized virus were tested for infectivity on HEK-293, COS7, NIH-3T3 and HeLa cells. Cells were analyzed 24h post-transduction and the number of GFP positive cells (%) was monitored by flow cytometry. The LV/RV-magnetic beads efficiently captured lentiviruses/retroviruses (blue bars), the supernatants (grey bars) are nearly non-infectious (absence of virus) whereas the concentrated virus bound to the beads are highly infectious.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com