Magnetic Antibody Purification System
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- All reagents (including Protein A magnetic beads) are ready-to-use as supplied.
- 20-200 µg of antibody can be purified in each run.
- Compatible with our Lightning-Link® Antibody Conjugation Kits and Oligonucleotide Conjugation Kit range.
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Supplier Data
Purification - Magnetic Antibody Purification System (AB270532)
Rabbit IgG purification.
20 μg (A), 25 μg (B) or 50 μg (C) rabbit IgG was purified using Magnetic Antibody Purification System (ab270532), demonstrating high recoveries in the first elution fraction even at the smallest working scale.
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Supplier Data
Purification - Magnetic Antibody Purification System (AB270532)
How does the kit work?.
The method involves capturing the antibody on the Protein A Magnetic Beads. Protein A has a high affinity for the Fc regions of IgG molecules from a variety of species; once the antibody has bound to the Protein A, unwanted substances can be removed by simply collecting the beads in a magnetic stand and washing the beads. The antibody is then eluted and neutralized.
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Supplier Data
Purification - Magnetic Antibody Purification System (AB270532)
Rabbit serum purification.
A sample of rabbit serum has been purified by using Magnetic Antibody Purification System (ab270532).
The gel picture shows how all the contaminants are effectively removed and the purified rabbit IgG is eluted.
Product details
Magnetic Antibody Purification System (ab270532) is a convenient method to purify antibodies at a small scale with high recoveries. It quickly removes substances such as BSA, glycine, tris and azide that interfere with conjugation reactions. It can be easily adapted to your needs allowing you to purify from 20 to 200 μg of antibody and to reach the desired final antibody concentration. The antibody to be purified or cleaned up should ideally be in a volume of 20 μl to 1 ml.
No dialysis of the antibody is needed prior to conjugation as interfering substances such as Tris, citrate and glycine have been replaced with conjugation-friendly alternatives.
This sytem can directly replace any existing protein A purification kit and can be used to isolate IgG antibodies from crude mixtures.
- Less than 15 minutes hands on time.
- 70-90% antibody recovery.
- Up to 10 purifications at the smallest working scale.
This product is manufactured by Expedeon, an Abcam company. It was previously called AbPure™ Universal Magnetic Purification System. Expedeon product code 265-0200 is the same as the 400 μL size of this product.
This product is manufactured by Expedeon, an Abcam company. It was previously called AbPure™ Universal Magnetic Purification System. Expedeon product code 265-0200 is the same as the 400 μL size of this product.
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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