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AB128745

Mouse Antibody Purification Kit

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(2 Publications)

Mouse Antibody Purification Kit (ab128745) is designed to remove substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores.

- All reagents (including resin) are ready-to-use as supplied.
- 20-150 µg of antibody can be purified in each run.
- Compatible with our Lightning-Link® Antibody Conjugation Kits and Oligonucleotide Conjugation Kit range.

Key facts

Reacts with

Mouse

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Product details

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The mouse antibody to be purified or cleaned up ideally is in a volume of 100 μL to 0.5 mL. 20 μg to 150 μg of antibody can be purified in each run.

The method involves capture of the antibody on a mouse resin and the removal of unwanted substances by a simple wash procedure, which is carried out in a standard microfuge. The purified product is then eluted and neutralized.

Note: Binding strength of Bovine IgG to the Mouse resin is negligable.

Antibodies purified using the Mouse Antibody Purification Kit are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our nanoparticle Antibody conjugation kits. To purify antibodies for use with our nanoparticle conjugation kits (Gold, Latex, Europium and Magnetic), please use our Mouse Antibody Purification Kit - Nanoparticles ab204910.

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ Mouse Antibody Purification System. 830-0010 is the same as the 3 tests size. 830-0005 is the same as the 1 test size.

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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 9: PubMed34579262

2021

Multicomponent Vaccines against Group A Streptococcus Can Effectively Target Broad Disease Presentations.

Applications

Unspecified application

Species

Unspecified reactive species

Helen A Shaw,James Ozanne,Keira Burns,Fatme Mawas

Immunological investigations 49:744-757 PubMed32799717

2020

An Immunosuppressive Effect of Melanoma-derived Exosomes on NY-ESO-1 Antigen-specific Human CD8 T Cells is Dependent on IL-10 and Independent of BRAF Mutation in Melanoma Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

ShinLa Shu,Junko Matsuzaki,Muzamil Y Want,Alexis Conway,Shawna Benjamin-Davalos,Cheryl L Allen,Marina Koroleva,Sebastiano Battaglia,Adekunle Odunsi,Hans Minderman,Marc S Ernstoff
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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