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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants.

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Reactive species

Mouse

What's included?

3 Test
Components
Additional Collecting Tubes
12 x 1 Vial
Binding Buffer (10X)
1 x 200 µL
Elution Buffer
1 x 1500 µL
Mouse Resin
3 x 400 µL
Neutralizer Buffer
1 x 500 µL
Spin cartridge/ collecting tube assemblies
3 x 1 Unit
Wash Buffer
1 x 12 mL

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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants.

Key facts

Reactive species

Mouse

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab128745 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The mouse antibody to be purified or cleaned up ideally is in a volume of 100 μL to 0.5 mL. 20 μg to 150 μg of antibody can be purified in each run.

The method involves capture of the antibody on a mouse resin and the removal of unwanted substances by a simple wash procedure, which is carried out in a standard microfuge. The purified product is then eluted and neutralized.

Note: Binding strength of Bovine IgG to the Mouse resin is negligable.

Antibodies purified using the Mouse Antibody Purification Kit are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our nanoparticle Antibody conjugation kits. To purify antibodies for use with our nanoparticle conjugation kits (Gold, Latex, Europium and Magnetic), please use our Mouse Antibody Purification Kit - Nanoparticles ab204910.

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ Mouse Antibody Purification System. 830-0010 is the same as the 3 tests size. 830-0005 is the same as the 1 test size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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