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Mouse Antibody Purification Kit - Nanoparticles (ab204910) removes contaminants often found in commercial antibodies.

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What's included?

3 Test
Components
Additional Collecting Tubes
12 x 1 Unit
Binding Buffer (10X)
1 x 250 µL
Elution Buffer
1 x 1500 µL
Mouse Resin
3 x 400 µL
Neutralizer
1 x 250 µL
Spin cartridge/ collecting tube assemblies
3 x 1 Unit
Wash Buffer
1 x 12 mL

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Mouse Antibody Purification Kit - Nanoparticles (ab204910) removes contaminants often found in commercial antibodies.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Mouse Antibody Purification Kit - Nanoparticles (ab204910) removes contaminants often found in commercial antibodies (e.g. BSA, glycine, tris and azide) that interfere in conjugation reactions with nanoparticles particles using our nanoparticle conjugation kits (Gold, Latex, Europium and Magnetic). It can also be used to purify mouse antibodies from crude samples such as ascites fluid. The purification system can be used with antibody volumes from 100-500 μL. 20 to 150 μg of antibody can be purified in each test.

The method involves capturing the mouse antibody on Mouse Resin and removing unwanted substances by a simple wash procedure, which is performed in a standard microfuge. The purified antibody is then eluted and neutralized.

Antibodies purified using the Mouse Antibody Purification Kit - Nanoparticles are fully compatible with our Gold antibody conjugation kits, Magnetic particle conjugation kits, Latex and Europium conjugation kits (available separately). To purify antibodies for use with our other conjugation kits, we recommend our Mouse Antibody Purification Kit (ab128745).

Notes:
(1) The Mouse Antibody Purification Kit - Nanoparticles is not suitable for use with antibodies from other species.
(2) The binding strength of bovine IgG to the Mouse Resin is negligible.

This product is manufactured by Expedeon, an Abcam company, and has previously been called AbPure™ Mouse Specific Purification System and GOLD mouse antibody purification kit. 261-0005 is the same as the 1 test size. 261-0010 is the same as the 3 tests size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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