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Efficient mouse antibody purification from tissue culture supernatants.

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Images

Functional Studies - Mouse TCS Antibody Purification Kit (AB128749), expandable thumbnail
  • SDS-PAGE - Mouse TCS Antibody Purification Kit (AB128749), expandable thumbnail

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What's included?

3 Test
Components
Binding Buffer (10X)
1 x 18 mL
Elution Buffer
1 x 15 mL
Mouse Resin
3 x 2 mL
Neutralizer Buffer
1 x 5 mL
Purification Column
3 x 1 Unit
Spin Cartridge / Collecting Tube Assemblies
3 x 1 Unit
Wash Buffer
1 x 75 mL

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Efficient mouse antibody purification from tissue culture supernatants.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Efficient mouse antibody purification from tissue culture supernatants. Processes 10-25 mL of supernatant to purify up to 1.5 mg of antibody per run.

The mouse resin has a very high affinity and specificity for mouse IgG molecules. ab128749 Mouse TCS Antibody Purification Kit can be used to purify mouse IgG fractions from hybridoma supernatants. The binding strength of bovine IgG to Abcam's Mouse resin is negligible; therefore, it can be used to selectively recover mouse IgG from TCS samples.

The method involves capture of the mouse antibody on the Mouse resin and removal of unwanted substances using a simple wash procedure. The purified product is eluted and neutralized.

Antibodies purified using the Mouse TCS Antibody Purification kit are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit, however they are not compatible with our nanoparticle Antibody conjugation kits. To purify antibodies for use with our nanoparticle conjugation kits (Gold, Latex, Europium and Magnetic), please use our Mouse Antibody Purification Kit - Nanoparticles Mouse Antibody Purification Kit (Gold/Europium labelling) ab204910.

ab128749 is suitable for use with Mouse antibodies. It is not suitable for use with antibodies from other species.

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ Mouse TCS Antibody Purification System. 832-0500 is the same as the 3 tests size. 832-0005 is the same as the 1 test size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Functional Studies - Mouse TCS Antibody Purification Kit (ab128749), expandable thumbnail

    Functional Studies - Mouse TCS Antibody Purification Kit (ab128749)

    A representative bar graph comparing the effectiveness of Mouse TCS Antibody Purification Kit and Protein G resin.
  • SDS-PAGE - Mouse TCS Antibody Purification Kit (ab128749), expandable thumbnail

    SDS-PAGE - Mouse TCS Antibody Purification Kit (ab128749)

    Gel electrophoresis of TCS samples purified with the Mouse TCS Antibody Purification Kit.

    M = Mouse IgG1 in a TCS sample

    B = Bovine serum IgG in a TCS sample

    TCS = Tissue culture supernatant sample

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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