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Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins.

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Sample type

Cell culture media, Serum, Ascites Fluid

Reactive species

Human, Mouse, Rat, Rabbit, Goat

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Reactivity data

Application
Purification
Reactivity
Reacts
Dilution info
-
Notes

-

Images

What's included?

10 Test
Components
Binding Buffer (10X)
1 x 40 mL
Elution Buffer (10X)
1 x 20 mL
Hi-Bind Protein Column
1 x 1 Unit
Neutralization Buffer
1 x 10 mL

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Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins.

Key facts

Sample type

Cell culture media, Serum, Ascites Fluid

Reactive species

Human, Mouse, Rat, Rabbit, Goat

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. Maximum IgG specificity is achieved by eliminating its cell wall binding region, albumin binding region, and other non-specific regions. Hi-BindTM Protein A-Agarose beads (Cat. No. 6520) for purification of IgG contain covalently coupled recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein purification. The Protein A Antibody Purification Kit is a simple, ready to use kit containing all necessary buffers and pre-packed columns to purify up to 350 mg of IgG. The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

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