Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins.
Cell culture media, Serum, Ascites Fluid
Human, Mouse, Rat, Rabbit, Goat
Application | Reactivity | Dilution info | Notes |
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Application Purification | Reactivity Reacts | Dilution info - | Notes - |
Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins.
Cell culture media, Serum, Ascites Fluid
Human, Mouse, Rat, Rabbit, Goat
Blue Ice
+4°C
+4°C
+4°C
Protein A beads have been widely used for IgG purification for their ability to bind immunoglobulins. Protein A is a genetically engineered protein containing five IgG-binding regions of native Protein A. Maximum IgG specificity is achieved by eliminating its cell wall binding region, albumin binding region, and other non-specific regions. Hi-BindTM Protein A-Agarose beads (Cat. No. 6520) for purification of IgG contain covalently coupled recombinant Protein A to 6% cross-linked Agarose beads, the most popular resin for protein purification. The Protein A Antibody Purification Kit is a simple, ready to use kit containing all necessary buffers and pre-packed columns to purify up to 350 mg of IgG. The kit can be used to purify antibodies in serum, ascites and cell culture medium from various species such as human, mouse, rat, goat and rabbit.
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Human IgG elution curve.
Protein A Antibody Purification Kit binding capacity was tested with 10 ml human serum. The sample was incubated overnight at 4°C. IgG amount as a function of order of eluted fractions.
SDS-PAGE of purified IgG from different species using Protein A Antibody Purification Kit (5 or 10 μg IgG per lane from 5 different species).
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