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Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.

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What's included?

3 Test
Components
Binding Buffer (10X)
1 x 18 mL
Elution Buffer
1 x 15 mL
Neutralizer Buffer
1 x 2 mL
Purification Column
3 x 1 Unit
Wash Buffer
1 x 75 mL

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Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.

The antibody is captured on the resin and unwanted substances are removed by a simple wash procedure. The purified product is then eluted and neutralized.

The components of ab109209 are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our nanoparticle Antibody conjugation kits (Gold, Latex, Europium and Magnetic). To purify antibodies for use with our nanoparticle conjugation kits, please use our Antibody Purification Kit - Nanoparticles (ab204909).

ab109209 is not suitable for goat antibody purification. Please refer to the protocol booklet for more information about suitable and unsuitable species antibodies.

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ A Serum Antibody Purification System. 863-0500 is the same as the 3 tests size. 863-0030 is the same as the 1 test size.

ab109209 contains 1-3 columns, and each can purify up to 20mg of antibody per run.

The volume of sample required will depend on the host species (See Table below):

SpeciesNormal range IgG(mg/ml)Suitable Volumes for Product(ml)
Rabbit12 - 151.3 - 1.7
Human7 - 230.9 - 2.9
Mouse2 - 54 - 10
Sheep/Goat18 - 240.8 - 1.1
Rat5 - 72.9 - 4
Ascites Fluid0.5 - 54 - 40

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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