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AB109209

Serum Antibody Purification Kit (Protein A)

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(2 Publications)

Serum Antibody Purification Kit (Protein A) (ab109209) is designed to purify IgG fractions from serum. The kit uses Protein A which has a high affinity for Fc regions of IgG molecules from a variety of species.

- All reagents (including Protein A resin) are ready-to-use as supplied.
- Up to 20 mg of antibody can be purified in each run.
- Compatible with our Lightning-Link® Antibody Conjugation Kits and Oligonucleotide Conjugation Kit range.

Key facts

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Product details

Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.

The antibody is captured on the resin and unwanted substances are removed by a simple wash procedure. The purified product is then eluted and neutralized.

The components of ab109209 are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our nanoparticle Antibody conjugation kits (Gold, Latex, Europium and Magnetic). To purify antibodies for use with our nanoparticle conjugation kits, please use our Antibody Purification Kit - Nanoparticles (ab204909).

ab109209 is not suitable for goat antibody purification. Please refer to the protocol booklet for more information about suitable and unsuitable species antibodies.

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ A Serum Antibody Purification System. 863-0500 is the same as the 3 tests size. 863-0030 is the same as the 1 test size.

ab109209 contains 1-3 columns, and each can purify up to 20mg of antibody per run.

The volume of sample required will depend on the host species (See Table below):

Species Normal range IgG(mg/ml) Suitable Volumes for Product(ml)
Rabbit 12 - 15 1.3 - 1.7
Human 7 - 23 0.9 - 2.9
Mouse 2 - 5 4 - 10
Sheep/Goat 18 - 24 0.8 - 1.1
Rat 5 - 7 2.9 - 4
Ascites Fluid 0.5 - 5 4 - 40
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What's included?

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Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical microbiology 60:e0023622 PubMed35652310

2022

Validation of an Indirect Immunofluorescence Assay and Commercial Q Fever Enzyme-Linked Immunosorbent Assay for Use in Macropods.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Tolpinrud,John Stenos,Anne-Lise Chaber,Joanne M Devlin,Catherine Herbert,An Pas,Magdalena Dunowska,Mark A Stevenson,Simon M Firestone

Inflammopharmacology 25:643-651 PubMed28528362

2017

Budesonide, fluticasone propionate, and azithromycin do not modulate the membrane vesicle release by THP-1 macrophages and respiratory pathogens during macrophage infection.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Volgers,Gert E Grauls,Pauline H M Hellebrand,Paul H M Savelkoul,Frank R M Stassen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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