Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.
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Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.
The antibody is captured on the resin and unwanted substances are removed by a simple wash procedure. The purified product is then eluted and neutralized.
The components of ab109209 are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our nanoparticle Antibody conjugation kits (Gold, Latex, Europium and Magnetic). To purify antibodies for use with our nanoparticle conjugation kits, please use our Antibody Purification Kit - Nanoparticles (Antibody Purification Kit (Gold/Europium labelling) ab204909).
ab109209 is not suitable for goat antibody purification. Please refer to the protocol booklet for more information about suitable and unsuitable species antibodies.
This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ A Serum Antibody Purification System. 863-0500 is the same as the 3 tests size. 863-0030 is the same as the 1 test size.
ab109209 contains 1-3 columns, and each can purify up to 20mg of antibody per run.
The volume of sample required will depend on the host species (See Table below):
| Species | Normal range IgG(mg/ml) | Suitable Volumes for Product(ml) |
|---|---|---|
| Rabbit | 12 - 15 | 1.3 - 1.7 |
| Human | 7 - 23 | 0.9 - 2.9 |
| Mouse | 2 - 5 | 4 - 10 |
| Sheep/Goat | 18 - 24 | 0.8 - 1.1 |
| Rat | 5 - 7 | 2.9 - 4 |
| Ascites Fluid | 0.5 - 5 | 4 - 40 |
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