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Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species.

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Sample type

Serum, Ascites Fluid

What's included?

3 Test
Components
Elution Buffer
1 x 15 mL
G Binding Buffer (10X)
1 x 18 mL
Neutralizer Buffer
1 x 5 mL
Purification Column
3 x 1 Unit
Serum Protein G Resin
3 x 4 mL
Wash Buffer
1 x 75 mL

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Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species.

Key facts

Sample type

Serum, Ascites Fluid

Storage

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. ab128751 G Serum resin is prepared by coupling purified Protein G to agarose beads. It can therefore be used to purify IgG fractions from both serum and ascites fluid. The antibody is captured on the AbSelect G resin and unwanted substances are removed by a simple wash procedure. Up to 10mg of antibody can be purified in each run. The volume of sample required will depend on the host species.

The purified product is then eluted and neutralized.

The antibodies purified with Protein G Serum Antibody Purification Kit are fully compatible with our Lightning-Link® antibody conjugation kits and Oligonucleotide Conjugation Kit. To purify antibodies for use with our nanoparticle conjugation kits, please use Antibody Purification Kit - Nanoparticles (ab204909).

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ G Serum Antibody Purification System. 893-0030 is the same as the 1 test size. 893-0500 is the same as the 3 tests size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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