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Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species.

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Key facts

Sample type
Serum, Ascites Fluid

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What's included?

3 Test
Components
Elution Buffer
1 x 15 mL
G Binding Buffer (10X)
1 x 18 mL
Neutralizer Buffer
1 x 5 mL
Purification Column
3 x 1 Unit
Serum Protein G Resin
3 x 4 mL
Wash Buffer
1 x 75 mL

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Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species.

Key facts

Sample type
Serum, Ascites Fluid

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. ab128751 G Serum resin is prepared by coupling purified Protein G to agarose beads. It can therefore be used to purify IgG fractions from both serum and ascites fluid. The antibody is captured on the AbSelect G resin and unwanted substances are removed by a simple wash procedure. Up to 10mg of antibody can be purified in each run. The volume of sample required will depend on the host species.

The purified product is then eluted and neutralized.

The antibodies purified with Protein G Serum Antibody Purification Kit are fully compatible with our Lightning-Link® antibody conjugation kits and Oligonucleotide Conjugation Kit. To purify antibodies for use with our nanoparticle conjugation kits, please use Antibody Purification Kit - Nanoparticles (Antibody Purification Kit (Gold/Europium labelling) ab204909).

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ G Serum Antibody Purification System. 893-0030 is the same as the 1 test size. 893-0500 is the same as the 3 tests size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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