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TCS Antibody Purification Kit (ab109207) has been designed for tissue culture supernatant purification, and can be used to purify 10-50 mL in each purification.

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What's included?

3 Test
Components
Binding Buffer (10X)
1 x 18 mL
Elution Buffer
1 x 15 mL
Neutralizer Buffer
1 x 5 mL
Protein A resin
3 x 2 mL
Purification Column
3 x 1 Unit
Spin cartridge/ collecting tube assemblies
3 x 1 Unit
Wash Buffer
1 x 75 mL

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TCS Antibody Purification Kit (ab109207) has been designed for tissue culture supernatant purification, and can be used to purify 10-50 mL in each purification.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

TCS Antibody Purification Kit (ab109207) has been designed for tissue culture supernatant purification, and can be used to purify 10-50 mL in each purification. Each column can purify up to 5 mg of antibody.

This kit works by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from hybridoma supernatants. The antibody is captured on the TCS resin and unwanted substances are removed by a simple wash procedure. The purified product is then eluted and neutralized.

The antibodies purified with the TCS Antibody Purification Kit are fully compatible with our Lightnign-Link® Antibody Conjugation kits and our Oligonucleotide Conjugation Kit. To purify antibodies from TCS for use with our nanoparticle conjugation kits, please use our TCS Antibody Purification Kit - Nanoparticles (ab270554).

ab109207 is not suitable for goat antibody purification.

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ A Antibody Purification System. 862-0500 is the same as the 3 tests size. 862-0030 is the same as the 1 test size.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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