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AB171529

450 nm Stop Solution for TMB Substrate

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(28 Publications)

450 nm Stop Solution for TMB Substrate is a ready to suse stop solution for colorimetric HRP-based ELISA detection.

- Designed to work with our range of TMB substrates
- For colorimetric readout at 450 nm

Key facts

Applications

ELISA

applications

Form

Liquid

form

Storage buffer

pH: 1 - 5 Constituents: 5% Methanesulphonic acid

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.</p>" } } }

Product details

Abcam's 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.

Product Use:
Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

Product protocols

Target data

Publications (28)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5359 PubMed40620807

2025

An Improved m6A-ELISA for Quantifying -methyladenosine in Poly(A)-purified mRNAs.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yee Chan,Waleed S Albihlal,Folkert J Van Werven

Frontiers in immunology 16:1565934 PubMed40458417

2025

Addressing SARS-CoV-2 evolution: neutralization of emerging variants of concern by the AVX/COVID-12 'Patria' vaccine based on HexaPro-S ancestral Wuhan spike or its updated BA.2.75.2 version.

Applications

Unspecified application

Species

Unspecified reactive species

Gregorio Carballo-Uicab,Gabriela Mellado-Sánchez,Edith González-González,Juana Salinas-Trujano,Ivette Mendoza-Salazar,Karina López-Olvera,Keyla M Gómez-Castellano,Ma Isabel Salazar,Jesús M Torres-Flores,Héctor Elías Chagoya-Cortés,Georgina Paz-De la Rosa,Ignacio Mena,Oscar Rojas-Martínez,Jesús Horacio Lara-Puente,Gustavo Javier Peralta-Sánchez,David Sarfati-Mizrahi,Alejandro Torres-Flores,Weina Sun,Florian Krammer,Adolfo García-Sastre,Peter Palese,Constantino López-Macías,Bernardo Lozano-Dubernard,Sonia M Pérez-Tapia,Juan C Almagro

Cell reports. Medicine 5:101754 PubMed39383869

2024

Activated sympathetic nerve post stroke downregulates Toll-like receptor 5 and disrupts the gut mucosal barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Huidi Wang,Jie Li,Guangyan Wu,Xiaofei Lin,Jiaying Chen,Jingru Liang,Jiahui Zhang,Xiaoxia Luo,Hongyun Mao,Jiahui Xie,Zhuang Li,Hongwei Zhou,Kaiyu Xu,Jia Yin,Yan He

Investigative ophthalmology & visual science 65:34 PubMed39028977

2024

Altered Elastin Turnover, Immune Response, and Age-Related Retinal Thinning in a Transgenic Mouse Model With RPE-Specific HTRA1 Overexpression.

Applications

Unspecified application

Species

Unspecified reactive species

Soumya Navneet,Masaaki Ishii,Bärbel Rohrer

Analytical chemistry 96:9676-9683 PubMed38813952

2024

Surface Acoustic Wave-Driven Enhancement of Enzyme-Linked Immunosorbent Assays: ELISAW.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Shuai Zhang,Cécile Floer,Sreeya Anjana Raj Kantubuktha,María José González Ruiz Velasco,James Friend

Vaccines 11: PubMed38140233

2023

Preliminary Study on the Efficacy of a Recombinant, Subunit SARS-CoV-2 Animal Vaccine against Virulent SARS-CoV-2 Challenge in Cats.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Morozov,Natasha N Gaudreault,Jessie D Trujillo,Sabarish V Indran,Konner Cool,Taeyong Kwon,David A Meekins,Velmurugan Balaraman,Bianca Libanori Artiaga,Daniel W Madden,Chester McDowell,Bradley Njaa,Jamie Retallick,Nicole Hainer,Jason Millership,William C Wilson,George Tkalcevic,Hanne Vander Horst,Yulia Burakova,Vickie King,Kendra Hutchinson,John M Hardham,Denise J Schwahn,Mahesh Kumar,Juergen A Richt

PloS one 18:e0287047 PubMed37315033

2023

Evaluation of 18F labeled glial fibrillary acidic protein binding nanobody and its brain shuttle peptide fusion proteins using a neuroinflammation rat model.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Morito,Ryuichi Harada,Ren Iwata,Yoichi Ishikawa,Nobuyuki Okamura,Yukitsuka Kudo,Shozo Furumoto,Kazuhiko Yanai,Manabu Tashiro

NPJ vaccines 8:31 PubMed36871059

2023

Immunogenicity and protective activity of mRNA vaccine candidates against yellow fever virus in animal models.

Applications

Unspecified application

Species

Unspecified reactive species

Lex G Medina-Magües,Janine Mühe,Edith Jasny,Emily S Medina-Magües,Nicole Roth,Jaime Lopera-Madrid,Cristhian Salas-Quinchucua,Cole Knuese,Benjamin Petsch,Jorge E Osorio

Biomaterials 292:121907 PubMed36436305

2022

Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge.

Applications

Unspecified application

Species

Unspecified reactive species

Si Sun,Entao Li,Gan Zhao,Jie Tang,Qianfei Zuo,Larry Cai,Chuanfei Xu,Cheng Sui,Yangxue Ou,Chang Liu,Haibo Li,Yuan Ding,Chao Li,Dongshui Lu,Weijun Zhang,Ping Luo,Ping Cheng,Yuwei Gao,Changchun Tu,Bruno Pitard,Joseph Rosenecker,Bin Wang,Yan Liu,Quanming Zou,Shan Guan

Viruses 14: PubMed36146773

2022

SARS-CoV-2 Spike and Nucleocapsid Antibody Response in Vaccinated Croatian Healthcare Workers and Infected Hospitalized Patients: A Single Center Cohort Study.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Kučan Brlić,Martina Pavletić,Mate Lerga,Fran Krstanović,Marina Pribanić Matešić,Karmela Miklić,Suzana Malić,Leonarda Mikša,Maja Pajcur,Dolores Peruč,Maren Schubert,Federico Bertoglio,Jurica Arapović,Alen Protić,Alan Šustić,Marko Milošević,Luka Čičin Šain,Stipan Jonjić,Vanda Juranić Lisnić,Ilija Brizić
View all publications

Product promise

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