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450 nm Stop Solution for TMB Substrate is a ready to suse stop solution for colorimetric HRP-based ELISA detection.

- Designed to work with our range of TMB substrates
- For colorimetric readout at 450 nm

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Key facts

Applications
ELISA
Form
Liquid

Reactivity data

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

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450 nm Stop Solution for TMB Substrate is a ready to suse stop solution for colorimetric HRP-based ELISA detection.

- Designed to work with our range of TMB substrates
- For colorimetric readout at 450 nm

Key facts

Applications
ELISA
Form
Liquid
Storage buffer

pH: 1 - 5
Constituents: 5% Methanesulphonic acid

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

Notes

Abcam's 450 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (TMB ELISA Substrate (Highest Sensitivity) ab171522, TMB ELISA Substrate (High Sensitivity) ab171523, TMB ELISA Substrate (Fast Kinetic Rate) ab171524, TMB ELISA Substrate (Slow Kinetic Rate) ab171525, TMB ELISA Substrate (Slower Kinetic Rate) ab171526 and TMB ELISA Substrate (Slowest Kinetic Rate) ab171527) and changes the chromogen from blue (620 nm) to yellow (450 nm) in color.

Product Use:
Equal volumes of TMB ELISA Substrate and 450 nm Stop Solution for TMB Substrate should be used.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com