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AB269260

ALDOA positive control ChIP primer pair

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(1 Publication)

Positive control ChIP-qPCR 5' and 3' primers for ALDOA gene.
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ChIP - ALDOA positive control ChIP primer pair (AB269260)
  • ChIP

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ChIP - ALDOA positive control ChIP primer pair (AB269260)

Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab32356 (red), and 20μl of Anti Rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.

Key facts

Applications

ChIP

applications

Form

Lyophilized

form

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Positive control ChIP-qPCR 5' and 3' primers for ALDOA gene. Use with SYBR green.

We recommend these primers as a positive control (based on Abcam's testing) for the Histone H3 dimethyl K4 histone mark. They may also be useful for other histone marks.

500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.

Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25μl.

Please contact us after purchase if you require the sequence of the oligos.

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 42:102048 PubMed40486495

2025

β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Zi-Long Wei,Shuo Han,Dong-Hua Han,Xue-Tao Li,Yu-Lun Huang,Zhi-Min Wang
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