Positive control ChIP-qPCR 5' and 3' primers for ALDOA gene.
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Key facts
- Applications
- ChIP
- Form
- Lyophilized
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ChIP | Reactivity Reacts | Dilution info - | Notes - |
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Positive control ChIP-qPCR 5' and 3' primers for ALDOA gene.
Key facts
- Applications
- ChIP
- Form
- Lyophilized
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Positive control ChIP-qPCR 5' and 3' primers for ALDOA gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the Histone H3 dimethyl K4 histone mark. They may also be useful for other histone marks.
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25μl.
Please contact us after purchase if you require the sequence of the oligos.
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1 product image
ChIP - ALDOA positive control ChIP primer pair (ab269260)
Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of Anti-Histone H3 (di methyl K4) antibody [Y47] - ChIP Grade ab32356 (red), and 20μl of Anti Rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach). Primers and probes are located in the first kb of the transcribed region.
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