Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent

Annexin V-Cy5 is a bright fluorescent reagent for detecting of the early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore serves as a probe for detecting apoptosis. Cy5 fluorescent dye produces an intense signal in the far-red region of the spectrum and therefore it is very useful for multiple labeling of cells with green and red colored fluorescent probes. Cy5 yields fluorescence with a λmax emission of 670 nm. Visit our for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called 1013 Annexin V-Cy5 Reagent.

Annexin V/ANXA5-Cy5 Apoptosis Detection Reagent ab14147 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

This Annexin V/ANXA5-Cy5 reagent can be used with Annexin V/ANXA5 binding buffer 10X Annexin V/ANXA5 Binding Buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-Cy5 reagent is also available as part of Annexin V/ANXA5-Cy5 apoptosis kit Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150.

For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.