Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
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Key facts
- Applications
- ICC/IF, Fluorescence Microscopy, Flow Cyt
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info 1 µL for 105 Cells | Notes - |
Application Fluorescence Microscopy | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
Key facts
- Applications
- ICC/IF, Fluorescence Microscopy, Flow Cyt
- Form
- Liquid
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 1% BSA
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The Annexin V-FITC conjugate can be used for detection of apoptosis by fluorescence microscopy or by flow cytometry. Visit our for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called 1001 Annexin V-FITC Reagent.
Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 (previously known as Annexin V-FITC Reagent 1001) can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
This Annexin V/ANXA5-FITC reagent can be used with Annexin V/ANXA5 binding buffer 10X Annexin V/ANXA5 Binding Buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-FITC reagent is also available as part of Annexin V/ANXA5-FITC apoptosis kit Annexin V-FITC Apoptosis Staining / Detection Kit ab14085.
For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.
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Image from Harper MT et al, J Biol Chem. 2010 Jun 25;285(26):19865-73. Epub 2010 Apr 13. DOI 10.1074/jbc.M109.085654Flow Cytometry - Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
Murine platelets were stimulated with CRP (5 μg/ml) in the presence of annexin V-fluorescein isothiocyanate for 10 minutes. The annexin V-positive population is indicated (AnV +ve).
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