Skip to main content

Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (AB14082), expandable thumbnail

Publications

Key facts

Applications
ICC/IF, Fluorescence Microscopy, Flow Cyt
Form
Liquid

Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
1 µL for 105 Cells
Notes

-

Application
Fluorescence Microscopy
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Recommended products

Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

Key facts

Applications
ICC/IF, Fluorescence Microscopy, Flow Cyt
Form
Liquid
Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 1% BSA

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The Annexin V-FITC conjugate can be used for detection of apoptosis by fluorescence microscopy or by flow cytometry. Visit our for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called 1001 Annexin V-FITC Reagent.

Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 (previously known as Annexin V-FITC Reagent 1001) can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

This Annexin V/ANXA5-FITC reagent can be used with Annexin V/ANXA5 binding buffer 10X Annexin V/ANXA5 Binding Buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-FITC reagent is also available as part of Annexin V/ANXA5-FITC apoptosis kit Annexin V-FITC Apoptosis Staining / Detection Kit ab14085.

For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.

Cell proliferation assays are indispensable for advancing scientific discovery and clinical applications. Browse our comprehensive selection to support your research.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry - Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082), expandable thumbnail
    Image from Harper MT et al, J Biol Chem. 2010 Jun 25;285(26):19865-73. Epub 2010 Apr 13. DOI 10.1074/jbc.M109.085654

    Flow Cytometry - Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)

    Murine platelets were stimulated with CRP (5 μg/ml) in the presence of annexin V-fluorescein isothiocyanate for 10 minutes. The annexin V-positive population is indicated (AnV +ve).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com