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Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

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Applications

ICC/IF, Fluorescence Microscopy, Flow Cyt

Form

Liquid

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Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
1 µL for 105 Cells
Notes

-

Application
Fluorescence Microscopy
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Images

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Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.

Key facts

Applications

ICC/IF, Fluorescence Microscopy, Flow Cyt

Form

Liquid

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 1% BSA

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The Annexin V-FITC conjugate can be used for detection of apoptosis by fluorescence microscopy or by flow cytometry. Visit our for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called 1001 Annexin V-FITC Reagent.

Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 (previously known as Annexin V-FITC Reagent 1001) can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

This Annexin V/ANXA5-FITC reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-FITC reagent is also available as part of Annexin V/ANXA5-FITC apoptosis kit ab14085.

For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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