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AB14082

Annexin V-FITC Apoptosis Detection Reagent (500X)

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(19 Publications)

Annexin V-FITC Apoptosis Detection Reagent ab14082 can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
1 Images
Flow Cytometry - Annexin V-FITC Apoptosis Detection Reagent (500X) (AB14082)
  • Flow Cyt

PubMed

Flow Cytometry - Annexin V-FITC Apoptosis Detection Reagent (500X) (AB14082)

Murine platelets were stimulated with CRP (5 μg/ml) in the presence of annexin V-fluorescein isothiocyanate for 10 minutes. The annexin V-positive population is indicated (AnV +ve).

Image from Harper MT et al, J Biol Chem. 2010 Jun 25;285(26):19865-73. Epub 2010 Apr 13. DOI 10.1074/jbc.M109.085654

Key facts

Applications

Flow Cyt, Fluorescence Microscopy, ICC/IF

applications

Form

Liquid

form

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 1% BSA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µL for 10^5 Cells", "notes":"<p></p>" }, "Fluorescence Microscopy": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 1001 Annexin V-FITC Reagent.

Annexin V-FITC Apoptosis Detection Reagent ab14082 (previously known as Annexin V-FITC Reagent 1001) can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.

This Annexin V-FITC reagent can be used with Annexin V binding buffer ab14084 (see protocol section for instructions). The Annexin V-FITC reagent is also available as part of Annexin V-FITC apoptosis kit ab14085.

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

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Target data

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 52: PubMed39329268

2024

miR‑25‑3p serves as an oncogenic in colorectal cancer cells by regulating the ubiquitin ligase FBXW7 function.

Applications

Unspecified application

Species

Unspecified reactive species

Yanbin Chen,Bingchen Chen,Shiliang Tu,Hang Yuan

Cell death & disease 13:431 PubMed35504885

2022

Somatic FOXC1 insertion mutation remodels the immune microenvironment and promotes the progression of childhood acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Wang,Xiaopeng Ma,Jie Huang,Xiaoyun Yang,Meiyun Kang,Xiaoyan Sun,Huimin Li,Yijun Wu,Heng Zhang,Yuting Zhu,Yao Xue,Yongjun Fang

American journal of cancer research 12:469-489 PubMed35261781

2022

Upregulation of Cyclin B2 () in breast cancer contributes to the development of lymphovascular invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Abrar I Aljohani,Michael S Toss,Khloud A El-Sharawy,Sameer Mirza,Graham R Ball,Andrew R Green,Emad A Rakha

Scientific reports 12:381 PubMed35013389

2022

GPR120 prevents colorectal adenocarcinoma progression by sustaining the mucosal barrier integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Rubbino,Valentina Garlatti,Valeria Garzarelli,Luca Massimino,Salvatore Spanò,Paolo Iadarola,Maddalena Cagnone,Martin Giera,Marieke Heijink,Simone Guglielmetti,Vincenzo Arena,Alberto Malesci,Luigi Laghi,Silvio Danese,Stefania Vetrano

Methods in molecular biology (Clifton, N.J.) 2445:139-169 PubMed34972991

2022

Approaches and Protocols to Analyze Autophagy and Its Role in Death of Apoptosis-Resistant Senescent Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Y Kochetkova,Valery A Pospelov,Tatiana V Pospelova

iScience 24:102807 PubMed34337362

2021

Identification of response signatures for tankyrase inhibitor treatment in tumor cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Line Mygland,Shoshy Alam Brinch,Martin Frank Strand,Petter Angell Olsen,Aleksandra Aizenshtadt,Kaja Lund,Nina Therese Solberg,Max Lycke,Tor Espen Thorvaldsen,Sandra Espada,Dorna Misaghian,Christian M Page,Oleg Agafonov,Ståle Nygård,Nai-Wen Chi,Eva Lin,Jenille Tan,Yihong Yu,Mike Costa,Stefan Krauss,Jo Waaler

Experimental physiology 105:2038-2050 PubMed33026112

2020

Inhibition of EZH2 attenuates coronary heart disease by interacting with microRNA-22 to regulate the TXNIP/nuclear factor-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Liu,Chuanzhong Dai,Yuping Lei,Wenzhen Wu,Wen Liu

Carcinogenesis 41:1421-1431 PubMed31917403

2020

Sildenafil triggers tumor lethality through altered expression of HSP90 and degradation of PKD2.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Chen,Yang Liu,Alexander Becher,Kristina Diepold,Evi Schmid,Adrian Fehn,Cornelia Brunner,Arefeh Rouhi,Gabriela Chiosis,Marcus Cronauer,Thomas Seufferlein,Ninel Azoitei

eLife 8: PubMed31682224

2019

Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yilin Kang,Alexander J Anderson,Thomas Daniel Jackson,Catherine S Palmer,David P De Souza,Kenji M Fujihara,Tegan Stait,Ann E Frazier,Nicholas J Clemons,Deidreia Tull,David R Thorburn,Malcolm J McConville,Michael T Ryan,David A Stroud,Diana Stojanovski

Metabolism: clinical and experimental 100:153962 PubMed31476350

2019

Endothelial senescence-associated secretory phenotype (SASP) is regulated by Makorin-1 ubiquitin E3 ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Sivareddy Kotla,Nhat-Tu Le,Hang Thi Vu,Kyung Ae Ko,Young Jin Gi,Tamlyn N Thomas,Carolyn Giancursio,Aldos J Lusis,John P Cooke,Keigi Fujiwara,Jun-Ichi Abe
View all publications
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Product promise

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