Annexin V-FITC Apoptosis Detection Reagent (500X)
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(19 Publications)
- Flow Cyt
PubMed
Flow Cytometry - Annexin V-FITC Apoptosis Detection Reagent (500X) (AB14082)
Murine platelets were stimulated with CRP (5 μg/ml) in the presence of annexin V-fluorescein isothiocyanate for 10 minutes. The annexin V-positive population is indicated (AnV +ve).
Image from Harper MT et al, J Biol Chem. 2010 Jun 25;285(26):19865-73. Epub 2010 Apr 13. DOI 10.1074/jbc.M109.085654
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Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The Annexin V-FITC conjugate can be used for detection of apoptosis by fluorescence microscopy or by flow cytometry. Visit our for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called 1001 Annexin V-FITC Reagent.
Annexin V-FITC Apoptosis Detection Reagent ab14082 (previously known as Annexin V-FITC Reagent 1001) can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
This Annexin V-FITC reagent can be used with Annexin V binding buffer ab14084 (see protocol section for instructions). The Annexin V-FITC reagent is also available as part of Annexin V-FITC apoptosis kit ab14085.
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Publications (19)
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Oncology reports 52: PubMed39329268
2024
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Cell death & disease 13:431 PubMed35504885
2022
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American journal of cancer research 12:469-489 PubMed35261781
2022
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Scientific reports 12:381 PubMed35013389
2022
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Methods in molecular biology (Clifton, N.J.) 2445:139-169 PubMed34972991
2022
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iScience 24:102807 PubMed34337362
2021
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Experimental physiology 105:2038-2050 PubMed33026112
2020
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Carcinogenesis 41:1421-1431 PubMed31917403
2020
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eLife 8: PubMed31682224
2019
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Metabolism: clinical and experimental 100:153962 PubMed31476350
2019
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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