Bradford reagent is an improved formulation for protein quantification, offering higher tolerance to detergent contamination and reduced protein-to-protein variation compared to traditional bradford assays.
- Cited in over 30 publications
- Excellent linearity across a defined range of protein concentrations
- Color Change – binds to protein, causing a shift in absorption from 465 nm to 595 nm with a color change from brown to blue
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- Nucleic acids research 49:4472-44922021Myod1 and GR coordinate myofiber-specific transcriptional enhancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela Rovito,Anna-Isavella Rerra,Vanessa Ueberschlag-Pitiot,Shilpy Joshi,Nezih Karasu,Vanessa Dacleu-Siewe,Khalil Ben Rayana,Kamar Ghaibour,Maxime Parisotto,Arnaud Ferry,Scott A Jelinsky,Gilles Laverny,Bruno P Klaholz,Tom Sexton,Isabelle M L Billas,Delphine Duteil,Daniel MetzgerPubMed 33836079
- Nature communications 11:62492020Cytosolic sequestration of the vitamin D receptor as a therapeutic option for vitamin D-induced hypercalcemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela Rovito et. al.PubMed 33288743
- Frontiers in cellular neuroscience 14:5555912020Inhibition of Brd4 by JQ1 Promotes Functional Recovery From Spinal Cord Injury by Activating Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Li et. al.PubMed 32982695
- Nature communications 9:14112018AKAPs-PKA disruptors increase AQP2 activity independently of vasopressin in a model of nephrogenic diabetes insipidus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFumiaki Ando et. al.PubMed 29650969
- Veterinary and comparative oncology 16:288-2962018Aberrant expression of microRNAs and the miR-1/MET pathway in canine hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesY-C Lai,N Ushio,M M Rahman,Y Katanoda,K Ogihara,Y Naya,A Moriyama,T Iwanaga,Y Saitoh,T Sogawa,T Sunaga,Y Momoi,H Izumi,N Miyoshi,Y Endo,M Fujiki,H Kawaguchi,N MiuraPubMed 29314614
- Biochemical and biophysical research communications 495:1655-16602017Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumiko I Matsuishi,Hiroki Kato,Keiji Masuda,Haruyoshi Yamaza,Yuta Hirofuji,Hiroshi Sato,Hiroko Wada,Tamotsu Kiyoshima,Kazuaki NonakaPubMed 29223396
- Nature communications 8:18192017Stem cell senescence drives age-attenuated induction of pituitary tumours in mouse models of paediatric craniopharyngioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJose Mario Gonzalez-Meljem et. al.PubMed 29180744
- Biotechnology for biofuels 10:2242017Removal of glucuronic acid from xylan is a strategy to improve the conversion of plant biomass to sugars for bioenergy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJan J Lyczakowski et. al.PubMed 28932265
- The international journal of biochemistry & cell biology 83:15-262016Bis(monoacylglycero)phosphate inhibits TLR4-dependent RANTES production in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Ciesielska,Hanna Sas-Nowosielska,Katarzyna KwiatkowskaPubMed 27939812
- Epigenetics & chromatin 9:332016Epigenetic dynamics of monocyte-to-macrophage differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Wallner et. al.PubMed 27478504
Key facts
- Form
- Liquid
Recommended products
Bradford reagent is an improved formulation for protein quantification, offering higher tolerance to detergent contamination and reduced protein-to-protein variation compared to traditional bradford assays.
- Cited in over 30 publications
- Excellent linearity across a defined range of protein concentrations
- Color Change – binds to protein, causing a shift in absorption from 465 nm to 595 nm with a color change from brown to blue
Key facts
- Form
- Liquid
- Storage buffer
Constituents: 14% Phosphoric acid, 3% Ethanol, 2.3% α-Cyclodextrin hydrate, 0.23% gamma-Cyclodextrin, 0.005% Brilliant Blue G
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
This product is manufactured by Expedeon, an Abcam company, and was previously called Bradford Ultra - 500 ml. BFU05L is the same as the 500 ml size.
Bradford Reagent ab119216 offers an improved formulation over classical Bradford: it has higher tolerance of detergent contamination of the protein samples.
This Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.
When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.
Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443.
Support on how to determine protein concentration can be found here.
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Functional Studies - Bradford Reagent (ab119216)
Functional Studies - Bradford Reagent (ab119216)
Functional Studies - Bradford Reagent (ab119216)
Functional Studies - Bradford Reagent (ab119216)
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