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Bradford reagent is an improved formulation for protein quantification, offering higher tolerance to detergent contamination and reduced protein-to-protein variation compared to traditional bradford assays.


- Cited in over 30 publications
- Excellent linearity across a defined range of protein concentrations
- Color Change – binds to protein, causing a shift in absorption from 465 nm to 595 nm with a color change from brown to blue
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Images

Functional Studies - Bradford Reagent (AB119216), expandable thumbnail
  • Functional Studies - Bradford Reagent (AB119216), expandable thumbnail
  • Functional Studies - Bradford Reagent (AB119216), expandable thumbnail
  • Functional Studies - Bradford Reagent (AB119216), expandable thumbnail

Publications

Key facts

Form
Liquid

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Bradford reagent is an improved formulation for protein quantification, offering higher tolerance to detergent contamination and reduced protein-to-protein variation compared to traditional bradford assays.


- Cited in over 30 publications
- Excellent linearity across a defined range of protein concentrations
- Color Change – binds to protein, causing a shift in absorption from 465 nm to 595 nm with a color change from brown to blue

Key facts

Form
Liquid
Storage buffer

Constituents: 14% Phosphoric acid, 3% Ethanol, 2.3% α-Cyclodextrin hydrate, 0.23% gamma-Cyclodextrin, 0.005% Brilliant Blue G

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

This product is manufactured by Expedeon, an Abcam company, and was previously called Bradford Ultra - 500 ml. BFU05L is the same as the 500 ml size.

Bradford Reagent ab119216 offers an improved formulation over classical Bradford: it has higher tolerance of detergent contamination of the protein samples.

This Bradford Reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.

When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to the absorbance obtained for a series of standard protein dilutions, which are assayed alongside samples with unknown protein concentrations.

Manufactured under patent numbers: US 12/226,797 ; SG 201103010-3 ; SG 200807953-5 ; JP 2009-507182 ; IN 5722/CHENP/2008 ; GB 0608377.8 ; EP 10187154.9; EP 07733647.7 ; CN 200780021434.8 ; CA 2,649,835 ; AU 2007245443.

Support on how to determine protein concentration can be found here.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Functional Studies - Bradford Reagent (ab119216), expandable thumbnail

    Functional Studies - Bradford Reagent (ab119216)

  • Functional Studies - Bradford Reagent (ab119216), expandable thumbnail

    Functional Studies - Bradford Reagent (ab119216)

  • Functional Studies - Bradford Reagent (ab119216), expandable thumbnail

    Functional Studies - Bradford Reagent (ab119216)

  • Functional Studies - Bradford Reagent (ab119216), expandable thumbnail

    Functional Studies - Bradford Reagent (ab119216)

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com