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AB269942

Carboxyl Gold Nanoparticles (40nm, 40OD)

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(2 Publications)

Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) are coated 40nm gold nanoparticles functionalized with carboxyl groups that allow the covalent conjugation of antibodies, using the water-soluble carbodiimide crosslinker EDC.

- Easy protocol – conjugate ready to use in less than 35 min, with no additional pre-activation or wash steps
- Proprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the gold – stable conjugates formed
- Uniform spherical shape and narrow size distribution – consistent high quality and batch to batch reproducibility
- Available in different formats to fit your experimental needs. Larger volume format available upon request
1 Images
Lateral flow assay - Carboxyl Gold Nanoparticles (40nm, 40OD) (AB269942)
  • LFA

Supplier Data

Lateral flow assay - Carboxyl Gold Nanoparticles (40nm, 40OD) (AB269942)

Lateral Flow Assay of Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) conjugated with EDC (1 step) to a monoclonal anti-CRP antibody titrated against CRP in buffer compared to a 2 steps EDC/NHS reaction using a competitor's 40nm carboxyl-gold. Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) shows a better sensitivity than the competitor, no aggregation is seen and requires only 35 minutes to make. Following the competitor's instruction, their product required from 3 to 5 hours for the EDC/NHS activation and conjugation reaction and significant optimization to prevent aggregation.

Key facts

Form

Liquid

form

Storage buffer

pH: 7

storage-buffer

Product details

Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) are coated gold nanoparticles functionalized with carboxyl groups that allow the covalent conjugation of antibodies, using the water-soluble carbodiimide crosslinker EDC.

Unlike traditional EDC/NHS coupling used to activate carboxyl groups, Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) are optimized for single step EDC covalent coupling without signs of aggregation. This eliminates the need for EDC/NHS pre-activation and washing steps associated with traditional conjugations.

This process is also significantly faster than standard methods, with conjugates ready to use in less than 35 minutes.

Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) have a narrow size distribution, a uniform spherical shape and high batch-to-batch consistency:

Mean Diameter 36-44 nm
SD <4 nm
CV% <10%

Product Details

Composition: 40nm carboxyl-gold 40 OD carboxyl gold nanoparticles
Size: 40 nm
Adsorption max: 530 nm
OD: 40.0
Particles per mL: 36 x 1011
Volume: 1 mL and 5 mL
Buffer: 10 mM EPPS, pH 8.5

Benefits of Carboxyl Gold Nanoparticles (40nm, 40 OD):

Easy protocol - conjugate ready to use in less than 35 min, with no additional pre-activation or wash steps

Proprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the gold-stable conjugates formed

Uniform spherical shape and narrow size distribution - consistent high quality and batch to batch reproducibility

Other Products
Check out our Gold Conjugation range here

This product is manufactured by Expedeon, an Abcam company. It was previously called 40nm InnovaCoat® GOLD - Carboxyl (40 OD). Expedeon productcode 3400-0001 is the same as the 40 µg size of this product and code 3400-0005 is the same as the 200 µg size of this product.

Properties and storage information

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Product protocols

Target data

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biosensors 14: PubMed38248407

2024

The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay.

Applications

Unspecified application

Species

Unspecified reactive species

Santosh Kumar Bikkarolla,Kavipriya Venkatesan,Yeddula Rebecca Revathy,Sowmya Parameswaran,Subramanian Krishnakumar,Dhananjaya Dendukuri

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference 2022:1428-1431 PubMed36086551

2022

Cystatin C as a biomarker for cardiorenal syndrome diseases quantitative diagnostics and monitoring via point-of-care.

Applications

Unspecified application

Species

Unspecified reactive species

X Zhang,S Fishlock,P Sharpe,J McLaughlin
View all publications
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