Carboxyl Gold Nanoparticles (40nm, 40OD)
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(2 Publications)
- Easy protocol – conjugate ready to use in less than 35 min, with no additional pre-activation or wash steps
- Proprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the gold – stable conjugates formed
- Uniform spherical shape and narrow size distribution – consistent high quality and batch to batch reproducibility
- Available in different formats to fit your experimental needs. Larger volume format available upon request
- LFA
Supplier Data
Lateral flow assay - Carboxyl Gold Nanoparticles (40nm, 40OD) (AB269942)
Lateral Flow Assay of Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) conjugated with EDC (1 step) to a monoclonal anti-CRP antibody titrated against CRP in buffer compared to a 2 steps EDC/NHS reaction using a competitor's 40nm carboxyl-gold. Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) shows a better sensitivity than the competitor, no aggregation is seen and requires only 35 minutes to make. Following the competitor's instruction, their product required from 3 to 5 hours for the EDC/NHS activation and conjugation reaction and significant optimization to prevent aggregation.
Product details
Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) are coated gold nanoparticles functionalized with carboxyl groups that allow the covalent conjugation of antibodies, using the water-soluble carbodiimide crosslinker EDC.
Unlike traditional EDC/NHS coupling used to activate carboxyl groups, Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) are optimized for single step EDC covalent coupling without signs of aggregation. This eliminates the need for EDC/NHS pre-activation and washing steps associated with traditional conjugations.
This process is also significantly faster than standard methods, with conjugates ready to use in less than 35 minutes.
Carboxyl Gold Nanoparticles (40nm, 40OD) (ab269942) have a narrow size distribution, a uniform spherical shape and high batch-to-batch consistency:
Mean Diameter 36-44 nm
SD <4 nm
CV% <10%
Product Details
Composition: 40nm carboxyl-gold 40 OD carboxyl gold nanoparticles
Size: 40 nm
Adsorption max: 530 nm
OD: 40.0
Particles per mL: 36 x 1011
Volume: 1 mL and 5 mL
Buffer: 10 mM EPPS, pH 8.5
Benefits of Carboxyl Gold Nanoparticles (40nm, 40 OD):
Easy protocol - conjugate ready to use in less than 35 min, with no additional pre-activation or wash steps
Proprietary surface coating prevents metal-protein interactions, and enables covalent attachment to the gold-stable conjugates formed
Uniform spherical shape and narrow size distribution - consistent high quality and batch to batch reproducibility
Other Products
Check out our Gold Conjugation range here
This product is manufactured by Expedeon, an Abcam company. It was previously called 40nm InnovaCoat® GOLD - Carboxyl (40 OD). Expedeon productcode 3400-0001 is the same as the 40 µg size of this product and code 3400-0005 is the same as the 200 µg size of this product.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
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Target data
Publications (2)
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Biosensors 14: PubMed38248407
2024
Applications
Unspecified application
Species
Unspecified reactive species
Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference 2022:1428-1431 PubMed36086551
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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