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Cresyl violet stain solution (0.1%) is a staining solution for visualizing Nissl substance in neurons on formalin-fixed, paraffin-embedded tissue.


- Stains Nissl granules violet and neuroglia nuclei slightly bluer
- Ideal for cerebral cortex tissue analysis
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cresyl Violet Stain Solution (0.1%) (AB246816), expandable thumbnail

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Key facts

Form
Liquid

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Cresyl violet stain solution (0.1%) is a staining solution for visualizing Nissl substance in neurons on formalin-fixed, paraffin-embedded tissue.


- Stains Nissl granules violet and neuroglia nuclei slightly bluer
- Ideal for cerebral cortex tissue analysis

Key facts

Form
Liquid
Storage buffer

pH: 2.9 - 3.3
Constituents: 0.2% Acetic acid, 0.1% 3,7-diamino-12H-benzo[b]phenoxazin-12-ium acetate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Cresyl Violet Stain Solution (0.1%) ab246816 is designed for staining Nissl substance in neurons on formalin fixed, paraffin-embedded tissue. Nissil granules are purple, nuclei of neuroglia and endothelial cells are slightly bluer than Nissl granules.

Nissil Substance: Violet.

Neuroglia Nuclei: Violet to Dark Blue.

Control Tissue: Cerebral Cortex

Procedure:

1. Deparaffinize sections and hydrate to distilled water.

2. Incubate slide in Cresyl Violet Stain Solution (0.1%) for 2-5 minutes.

3. Rinse quickly in 1 change of distilled water.

4. Dehydrate quickly in absolute alcohol. (Alcohol may remove stain from tissue over time).

5. Clear in Xylene or Xylene substitute and mount in synthetic resin.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cresyl Violet Stain Solution (0.1%) (ab246816), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cresyl Violet Stain Solution (0.1%) (ab246816)

    ab246816 Cresyl Violet Stain Solution (0.1%) staining formalin-fixed-paraffin embedded human brain.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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