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Cresyl Violet Stain Solution (1%) ab246817 is designed for staining Nissl substance in neurons on formalin fixed, paraffin-embedded tissue.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cresyl Violet Stain Solution (1%) (AB246817), expandable thumbnail

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Key facts

Form
Liquid

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Cresyl Violet Stain Solution (1%) ab246817 is designed for staining Nissl substance in neurons on formalin fixed, paraffin-embedded tissue.

Key facts

Form
Liquid
Storage buffer

pH: 3 - 3.4
Constituents: 1% 3,7-diamino-12H-benzo[b]phenoxazin-12-ium acetate, 0.2% Acetic acid

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Cresyl Violet Stain Solution (1%) ab246817 is designed for staining Nissl substance in neurons on formalin fixed, paraffin-embedded tissue. Nissl granules are purple, nuclei of neuroglia and endothelial cells are slightly bluer than Nissl granules.

Nissl Substance: Violet.

Neuroglia Nuclei: Violet to Dark Blue.

Control Tissue: Cerebral Cortex

Procedure:

Working Solutions: If preferred, reagent may be diluted up to 1:10 with Deionized water just before use.

1. Deparaffinize sections and hydrate to distilled water.

2. Apply Cresyl Violet Acetate solution (or working solution) to tissue for 3-5 minutes

3. Rinse quickly in 1 change of distilled water.

4. Dehydrate quickly in absolute alcohol. (Alcohol may remove stain from tissue over time).

5. Clear in Xylene or Xylene substitute and mount in synthetic resin.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cresyl Violet Stain Solution (1%) (ab246817), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cresyl Violet Stain Solution (1%) (ab246817)

    ab246817 Cresyl Violet Stain Solution (1%) staining formalin-fixed-paraffin embedded human brain.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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