Cresyl Violet Stain Solution (1%)

Product details

Cresyl Violet Stain Solution (1%) ab246817 is designed for staining Nissl substance in neurons on formalin fixed, paraffin-embedded tissue. Nissl granules are purple, nuclei of neuroglia and endothelial cells are slightly bluer than Nissl granules.

Nissl Substance: Violet.

Neuroglia Nuclei: Violet to Dark Blue.

Control Tissue: Cerebral Cortex

Procedure:

Working Solutions: If preferred, reagent may be diluted up to 1:10 with Deionized water just before use.

1. Deparaffinize sections and hydrate to distilled water.

2. Apply Cresyl Violet Acetate solution (or working solution) to tissue for 3-5 minutes

3. Rinse quickly in 1 change of distilled water.

4. Dehydrate quickly in absolute alcohol. (Alcohol may remove stain from tissue over time).

5. Clear in Xylene or Xylene substitute and mount in synthetic resin.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.