Positive control ChIP-qPCR 5' and 3' primers for GAPDH gene.
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Key facts
- Applications
- ChIP
- Form
- Lyophilized
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ChIP | Reactivity Reacts | Dilution info - | Notes - |
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Positive control ChIP-qPCR 5' and 3' primers for GAPDH gene.
Key facts
- Applications
- ChIP
- Form
- Lyophilized
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Positive control ChIP-qPCR 5' and 3' primers for GAPDH gene. Use with SYBR green.
We recommend these primers as a positive control (based on Abcam's testing) for the histone marks below. They may also be useful for other histone marks.
- Histone H3 acetyl K27
- Histone H3 tri methyl K4
- Histone H3 acetyl K9
- Histone H3 acetyl K18
- Histone H3 acetyl K4
- Histone H2A.Z
- Histone H3.3
- Histone H4 (unmodified)
500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.
Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25µl.
Please contact us after purchase if you require the sequence of the oligos.
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2 product images
ChIP - GAPDH positive control ChIP primer pair (ab267832)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 μg of chromatin, 5 μg of ab1012 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
ChIP - GAPDH positive control ChIP primer pair (ab267832)
Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade ab177178 (blue), and 20μl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads as a control sample (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
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