Hydrogen peroxide blocking reagent is used to block endogenous peroxidase activity in IHC applications, enhancing signal detection in HRP / peroxidase-based systems.
- Cited in over 30 publications
- Endogenous peroxidase block – prevents non-specific background staining in IHC
- HRP-based detection – ideal for use with HRP/peroxidase detection systems
- Concentration – contains 0.3% hydrogen peroxide
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Publications
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- Nature communications 14:65692023Phagocytosis-initiated tumor hybrid cells acquire a c-Myc-mediated quasi-polarization state for immunoevasion and distant dissemination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Wei Chou,Chia-Nung Hung,Cheryl Hsiang-Ling Chiu,Xi Tan,Meizhen Chen,Chien-Chin Chen,Moawiz Saeed,Che-Wei Hsu,Michael A Liss,Chiou-Miin Wang,Zhao Lai,Nathaniel Alvarez,Pawel A Osmulski,Maria E Gaczynska,Li-Ling Lin,Veronica Ortega,Nameer B Kirma,Kexin Xu,Zhijie Liu,Addanki P Kumar,Josephine A Taverna,Gopalrao V N Velagaleti,Chun-Liang Chen,Zhao Zhang,Tim Hui-Ming HuangPubMed 37848444
- Bioengineering (Basel, Switzerland) 10:2023Early Degenerative Changes in a Spontaneous Osteoarthritis Model Assessed by Nanoindentation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Davis,Jurgita Zekonyte,Aikaterina Karali,Marta Roldo,Gordon BlunnPubMed 37760097
- Arthritis & rheumatology (Hoboken, N.J.) 75:1573-15852023V-Set Immunoglobulin Domain-Containing Protein 4 as a Novel Serum Biomarker of Lupus Nephritis and Renal Pathology Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenling Tang,Shu Zhang,Aygun Teymur,Bowen Yang,Fariz Nazir,Qi Cai,Ramesh Saxena,Nancy J Olsen,Chandra Mohan,Tianfu WuPubMed 37163449
- Bone research 11:342023A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Wei,Zewen Kelvin Tuong,Mahmoud Omer,Christopher Ngo,Jackson Asiatico,Michael Kinzel,Abinaya Sindu Pugazhendhi,Annette R Khaled,Ranajay Ghosh,Melanie CoathupPubMed 37385982
- Science advances 9:eabq75952023Translational imaging of TSPO reveals pronounced innate inflammation in human and murine CD8 T cell-mediated limbic encephalitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Gallus,Wolfgang Roll,Andre Dik,Cristina Barca,Bastian Zinnhardt,Gordon Hicking,Christoph Mueller,Venu Narayanan Naik,Max Anstötz,Julia Krämer,Leoni Rolfes,Lydia Wachsmuth,Julika Pitsch,Karen M J van Loo,Saskia Räuber,Hideho Okada,Catriona Wimberley,Christine Strippel,Kristin S Golombeck,Andreas Johnen,Stjepana Kovac,Catharina C Groß,Philipp Backhaus,Robert Seifert,Jan Lewerenz,Rainer Surges,Christian E Elger,Heinz Wiendl,Tobias Ruck,Albert J Becker,Cornelius Faber,Andreas H Jacobs,Jan Bauer,Sven G Meuth,Michael Schäfers,Nico MelzerPubMed 37294768
- STAR protocols 4:1022642023Protocol for generation of multicellular spheroids through reduced gravity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDylan A Zinn,Christine Mehner,Tushar PatelPubMed 37133991
- Journal of the Association for Research in Otolaryngology : JARO 24:239-2512023Untargeted Metabolomic Analysis in Endolymphatic Sac Luminal Fluid from Patients with Meniere's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Huang,Qin Wang,Chao Huang,Zhou Zhou,Anquan Peng,Zhiwen ZhangPubMed 36715893
- Bioactive materials 21:547-5652022A novel approach for the prevention of ionizing radiation-induced bone loss using a designer multifunctional cerium oxide nanozyme.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Wei,Craig J Neal,Tamil Selvan Sakthivel,Yifei Fu,Mahmoud Omer,Amitava Adhikary,Samuel Ward,Khoa Minh Ta,Samuel Moxon,Marco Molinari,Jackson Asiatico,Michael Kinzel,Sergey N Yarmolenko,Vee San Cheong,Nina Orlovskaya,Ranajay Ghosh,Sudipta Seal,Melanie CoathupPubMed 36185749
- International journal of molecular sciences 23:2022Nebulized Non-Immunogenic Staphylokinase in the Mice Acute Lung Injury Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergey S Markin,Roman D Lapshin,Olga S Baskina,Svetlana A Korotchenko,Irina V Mukhina,Sergei V Ivanov,Mikhail P Semenov,Valerii V Beregovykh,Andrey M SemenovPubMed 36012579
- International journal of molecular sciences 23:2022Inhaled Placental Mesenchymal Stromal Cell Secretome from Two- and Three-Dimensional Cell Cultures Promotes Survival and Regeneration in Acute Lung Injury Model in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVasily A Kudinov,Rafael I Artyushev,Irina M Zurina,Elena S Zorina,Roman D Lapshin,Ludmila B Snopova,Irina V Mukhina,Irina N SaburinaPubMed 35408778
Key facts
- Applications
- IHC-P, IHC-Fr
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Hydrogen peroxide blocking reagent is used to block endogenous peroxidase activity in IHC applications, enhancing signal detection in HRP / peroxidase-based systems.
- Cited in over 30 publications
- Endogenous peroxidase block – prevents non-specific background staining in IHC
- HRP-based detection – ideal for use with HRP/peroxidase detection systems
- Concentration – contains 0.3% hydrogen peroxide
Key facts
- Applications
- IHC-P, IHC-Fr
- Form
- Liquid
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.3% Hydrogen peroxide
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Hydrogen Peroxide Blocking Reagent ab64218 for use in IHC with detection based on HRP / peroxidase.
Note: The concentration of hydrogen peroxide in this product is 0.3%.
IHC protocol suitable for use with Hydrogen Peroxide Blocking Reagent:
For frozen sections, skip steps 1 and 2.
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution ab64218 to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in antibody diluent and incubate.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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1 product image
Selby M.J et al., PLoS One 11(9), Fig 2. doi: 10.1371/journal.pone.0161779. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Frozen sections) - Hydrogen Peroxide Blocking Reagent (ab64218)
Immunohistochemical (frozen) analysis of MC38 tumor sections labeling CD3 with Anti-CD3 epsilon antibody [SP7] ab16669 at 1/400 dilution. Sections were fixed with acetone, treated with peroxidase block (ab64218) to quench endogenous peroxidase, and then further blocked with a 10% goat serum and 5% BSA solution. CD3 positive T cells were detected using ab80437. Invasive margin (top) shows sections derived from the periphery of the tumor and interior (bottom) shows sections from within the tumor.
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