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AB286846

Lyophilized Exosome Standard (100 µg, Human Serum)

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This product is manufactured by BioVision, an Abcam company and was previously called M1043 ExoStd™ Lyophilized Exosome Standard (100 μg, Human Serum, 2 vials).
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Functional Studies - Lyophilized Exosome Standard (100 µg, Human Serum) (AB286846)
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Supplier Data

Functional Studies - Lyophilized Exosome Standard (100 µg, Human Serum) (AB286846)

Comparative Nanosight analysis of freshly purified (lower panel) and lyophilized plasma exosomes (upper panel).

Flow Cytometry - Lyophilized Exosome Standard (100 µg, Human Serum) (AB286846)
  • Flow Cyt

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Flow Cytometry - Lyophilized Exosome Standard (100 µg, Human Serum) (AB286846)

Lyophilization is the ideal method for preserving exosome stability. Expression of exosomal markers was assayed with different techniques (WB, FACS, ELISA) and nanovesicle count was measured with NTA. Lyophilization did not substantially affect exosome count or biomarker expression compared to other storage methods.

Comparison of exosomal markers on fresh and lyophilized on fresh (F), frozen (-20C) and lyophilized exosomes (L).

Functional Studies - Lyophilized Exosome Standard (100 µg, Human Serum) (AB286846)
  • FuncS

Supplier Data

Functional Studies - Lyophilized Exosome Standard (100 µg, Human Serum) (AB286846)

Comparative detection of CD81 on exosomes. 1- Fresh exosomes; 2- Frozen exosomes (-20°C); 3- Lyophilized exosomes.

Western blot - Lyophilized Exosome Standard (100 µg, Human Serum) (AB286846)
  • WB

Supplier Data

Western blot - Lyophilized Exosome Standard (100 µg, Human Serum) (AB286846)

Expression of exosomal markers was assayed with different techniques (WB, FACS, ELISA) and nanovesicle count was measured with NTA. Lyophilization did not substantially affect exosome count or biomarker expression compared to other storage methods.

Western Blot comparison of exosomal markers on fresh (F), frozen (-20C) and lyophilized exosomes (L).

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Key facts

Form

Lyophilized

form

Product details

This product is manufactured by BioVision, an Abcam company and was previously called M1043 ExoStd™ Lyophilized Exosome Standard (100 μg, Human Serum, 2 vials). M1043-2 is the same size as the 2 vial size of ab286846.

This Lyophilized Exosome Standard (100 μg, Human Serum) ab286846 was previously known as ExoStd Lyophilized Exosome Standard (100 μg, Human Serum) M1043-2.

These lyophilized exosome standards are standardized positive controls for immunocapture performance evaluation. Lyophilization is the ideal technique for preserving the long-term stability of exosomes at 4°C. Lyophilized exosomes can be used as control standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples. Lyophilized exosomes are easy to ship and store, and are stable for over 36 months at 4°C. Purified and lyophilized exosomes are obtained from a variety of biological sources: cell culture supernatant, human plasma, serum, urine and saliva. Exosomes are purified following a combination of ultracentrifugation and microfiltration steps. Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis) with NanoSight LM10. Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids). These lyophilized exosome standards are highly pure, easy to reconstitute and easy to ship and store at 4°C.

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

Target data

websiteProtocolBooklet
en

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