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Lyophilized Exosome Standard (100 μg, Human Urine) ab286884 was previously known as ExoStd Lyophilized Exosome Standard (100 μg, Human Urine M1045-2.

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Images

Functional Studies - Lyophilized Exosome Standard (100 µg, Human Urine) (AB286884), expandable thumbnail
  • Flow Cytometry - Lyophilized Exosome Standard (100 µg, Human Urine) (AB286884), expandable thumbnail
  • Functional Studies - Lyophilized Exosome Standard (100 µg, Human Urine) (AB286884), expandable thumbnail
  • Western blot - Lyophilized Exosome Standard (100 µg, Human Urine) (AB286884), expandable thumbnail

Key facts

Form
Lyophilized

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Lyophilized Exosome Standard (100 μg, Human Urine) ab286884 was previously known as ExoStd Lyophilized Exosome Standard (100 μg, Human Urine M1045-2.

Key facts

Form
Lyophilized

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Avoid freeze / thaw cycle

Notes

Lyophilized Exosome Standard (100 μg, Human Urine) ab286884 was previously known as ExoStd Lyophilized Exosome Standard (100 μg, Human Urine M1045-2.

These lyophilized exosome standards are standardized positive controls for immunocapture performance evaluation. Lyophilization is the ideal technique for preserving the long-term stability of exosomes at 4°C. Lyophilized exosomes can be used as control standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples. Lyophilized exosomes are easy to ship and store, and are stable for over 36 months at 4°C. Purified and lyophilized exosomes are obtained from a variety of biological sources: cell culture supernatant, human plasma, serum, urine and saliva. Exosomes are purified following a combination of ultracentrifugation and microfiltration steps. Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis) with NanoSight LM10. Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids). These lyophilized exosome standards are highly pure, easy to reconstitute and easy to ship and store at 4°C.

For the efficient and convenient cleavage of recombinant fusion proteins containing Precission cleavage sequence (Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro).

Product promise

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4 product images

  • Functional Studies - Lyophilized Exosome Standard (100 µg, Human Urine) (ab286884), expandable thumbnail

    Functional Studies - Lyophilized Exosome Standard (100 µg, Human Urine) (ab286884)

    Comparative Nanosight analysis of freshly purified (lower panel) and lyophilized plasma exosomes (upper panel).

  • Flow Cytometry - Lyophilized Exosome Standard (100 µg, Human Urine) (ab286884), expandable thumbnail

    Flow Cytometry - Lyophilized Exosome Standard (100 µg, Human Urine) (ab286884)

    Lyophilization is the ideal method for preserving exosome stability. Expression of exosomal markers was assayed with different techniques (WB, FACS, ELISA) and nanovesicle count was measured with NTA. Lyophilization did not substantially affect exosome count or biomarker expression compared to other storage methods.

    Comparison of exosomal markers on fresh and lyophilized on fresh (F), frozen (-20C) and lyophilized exosomes (L).

  • Functional Studies - Lyophilized Exosome Standard (100 µg, Human Urine) (ab286884), expandable thumbnail

    Functional Studies - Lyophilized Exosome Standard (100 µg, Human Urine) (ab286884)

    Comparative detection of CD81 on exosomes. 1- Fresh exosomes; 2- Frozen exosomes (-20°C); 3- Lyophilized exosomes.

  • Western blot - Lyophilized Exosome Standard (100 µg, Human Urine) (ab286884), expandable thumbnail

    Western blot - Lyophilized Exosome Standard (100 µg, Human Urine) (ab286884)

    Expression of exosomal markers was assayed with different techniques (WB, FACS, ELISA) and nanovesicle count was measured with NTA. Lyophilization did not substantially affect exosome count or biomarker expression compared to other storage methods.

    Western Blot comparison of exosomal markers on fresh (F), frozen (-20C) and lyophilized exosomes (L).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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