Lyophilized Exosome Standard (100 µg, Human Urine)
Be the first to review this product! Submit a review
|
(0 Publication)
- Control (spike-in) for EV quantification, protein marker analysis (ELISA, Western blot, Flow cytometry).
- Extraction and analysis of EV associated nucleic acids (qPCR, ddPCR, RNA seq) and NTA, fluorescent NTA (F-NTA).
- Single EV analysis by electron microscopy (TEM), Fluorescent microscopy.
- Number of particles in 100 µg: > 1x1010
- FuncS
Supplier Data
Functional Studies - Lyophilized Exosome Standard (100 µg, Human Urine) (AB286884)
Comparative Nanosight analysis of freshly purified (lower panel) and lyophilized plasma exosomes (upper panel).
- Flow Cyt
Supplier Data
Flow Cytometry - Lyophilized Exosome Standard (100 µg, Human Urine) (AB286884)
Lyophilization is the ideal method for preserving exosome stability. Expression of exosomal markers was assayed with different techniques (WB, FACS, ELISA) and nanovesicle count was measured with NTA. Lyophilization did not substantially affect exosome count or biomarker expression compared to other storage methods.
Comparison of exosomal markers on fresh and lyophilized on fresh (F), frozen (-20C) and lyophilized exosomes (L).
- FuncS
Supplier Data
Functional Studies - Lyophilized Exosome Standard (100 µg, Human Urine) (AB286884)
Comparative detection of CD81 on exosomes. 1- Fresh exosomes; 2- Frozen exosomes (-20°C); 3- Lyophilized exosomes.
- WB
Supplier Data
Western blot - Lyophilized Exosome Standard (100 µg, Human Urine) (AB286884)
Expression of exosomal markers was assayed with different techniques (WB, FACS, ELISA) and nanovesicle count was measured with NTA. Lyophilization did not substantially affect exosome count or biomarker expression compared to other storage methods.
Western Blot comparison of exosomal markers on fresh (F), frozen (-20C) and lyophilized exosomes (L).
false
Complementary Products
Sample Prep & Detection Kits
AB267478
Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9)
- 0
- 0
Product details
Lyophilized Exosome Standard (100 μg, Human Urine) ab286884 was previously known as ExoStd Lyophilized Exosome Standard (100 μg, Human Urine M1045-2.
These lyophilized exosome standards are standardized positive controls for immunocapture performance evaluation. Lyophilization is the ideal technique for preserving the long-term stability of exosomes at 4°C. Lyophilized exosomes can be used as control standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples. Lyophilized exosomes are easy to ship and store, and are stable for over 36 months at 4°C. Purified and lyophilized exosomes are obtained from a variety of biological sources: cell culture supernatant, human plasma, serum, urine and saliva. Exosomes are purified following a combination of ultracentrifugation and microfiltration steps. Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis) with NanoSight LM10. Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids). These lyophilized exosome standards are highly pure, easy to reconstitute and easy to ship and store at 4°C.
For the efficient and convenient cleavage of recombinant fusion proteins containing Precission cleavage sequence (Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro).
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com