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AB288118

Lyophilized Exosome Standard (Human Plasma)

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Lyophilized Exosome Standard (100 µg, Human Plasma) (ab288118) contains purified exosomes (small-EVs) and available in 2x 100 µg format. Suitable to use in the following applications:

- Control (spike-in) for EV quantification, protein marker analysis (ELISA, Western blot, Flow cytometry).
- Extraction and analysis of EV associated nucleic acids (qPCR, ddPCR, RNA seq) and NTA, fluorescent NTA (F-NTA).
- Single EV analysis by electron microscopy (TEM), Fluorescent microscopy.
- Number of particles in 100 µg: > 1x1010
2 Images
Flow Cytometry - Lyophilized Exosome Standard (Human Plasma) (AB288118)
  • Flow Cyt

Supplier Data

Flow Cytometry - Lyophilized Exosome Standard (Human Plasma) (AB288118)

Comparison of exosomal markers on fresh and lyophilized exosomes (L).

Western blot - Lyophilized Exosome Standard (Human Plasma) (AB288118)
  • WB

Supplier Data

Western blot - Lyophilized Exosome Standard (Human Plasma) (AB288118)

Western Blot comparison of exosomal markers on fresh (F), frozen (-20C) and lyophilized exosomes (L).

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Key facts

Applications

Biochemical assay

applications

Reconstitution

Reconstitute in 100 µL of water

Form

Lyophilized

form

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Biochemical assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

Product details

This Lyophilized Exosome Standard (100 μg, Human Plasma) ab288118 was previously known as ExoStd Lyophilized Exosome Standard (100 μg, Human Plasma) M1041.

These lyophilized exosome standards are standardized positive controls for immunocapture performance evaluation. Lyophilization is the ideal technique for preserving the long-term stability of exosomes at 4 °C. Lyophilized exosomes can be used as control standards for multiple applications including FACS, WB, ELISA and as calibration standards for quantitation of exosome-derived markers from biological samples. Lyophilized exosomes are easy to ship and store, and are stable for over 36 months at 4 °C. Purified and lyophilized exosomes are obtained from a variety of biological sources: cell culture supernatant, human plasma, serum, urine and saliva. Exosomes are purified following a combination of ultracentrifugation and microfiltration steps. Exosomes are subsequently quantified and validated for overall protein content and particle number by NTA (Nanoparticles Tracking Analysis) with NanoSight LM10. Lyophilization does not affect the stability of purified exosomes and the expression levels of their exosome markers (proteins and nucleic acids). These lyophilized exosome standards are highly pure, easy to reconstitute and easy to ship and store at 4°C.

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Avoid freeze / thaw cycle

Product protocols

Target data

Product promise

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