Lyophilized MCF7 Exosome Standards

• Flow Cyt

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Flow Cytometry - Lyophilized MCF7 Exosome Standards (AB239691)

Relationship between background noise and specific signal at different exosome concentrations. Exosomes were captured by CD63+ (Clone TEA3/18) capture beads and subsequently detected by Anti-CD9 PE (Clone VJ1/20).

• sELISA

Supplier Data

Sandwich ELISA - Lyophilized MCF7 Exosome Standards (AB239691)

Interpolated concentration of native Human CD63 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Purified MCF-7 exosome standards (ab239691) 25 µg/mL (dilutions made into Sample Diluent NS) and denatured MCF-7 exosome standards (ab239691) 2.5 µg/mL (dilutions made in Sample Diluent NS + Enhancer). The interpolated dilution factor corrected values as a ratio of µg/mL exosome load are plotted (mean +/- SD, n=2).

• WB

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Western blot - Lyophilized MCF7 Exosome Standards (AB239691)

Fresh and lyophilized Exosome batches were analyzed and compared by WB in native conditions for exosomal markers, by anti-CD9 (Clone VJ1/20) and anti-CD63 (Clon TEA3/18) antibodies at a 1 : 1000 dilution (0.1 mg/ml).

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