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Positive control ChIP-qPCR 5' and 3' primers for Myo-D gene.

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Images

ChIP - Myo-D positive control ChIP primer pair (AB269261), expandable thumbnail
  • ChIP - Myo-D positive control ChIP primer pair (AB269261), expandable thumbnail
  • ChIP - Myo-D positive control ChIP primer pair (AB269261), expandable thumbnail

Key facts

Applications

ChIP

Form

Lyophilized

Reactivity data

Application

ChIP

Reactivity

Reacts

Dilution info

-

Notes

-

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Positive control ChIP-qPCR 5' and 3' primers for Myo-D gene.

Key facts

Applications

ChIP

Form

Lyophilized

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Positive control ChIP-qPCR 5' and 3' primers for Myo-D gene. Use with SYBR green.

We recommend these primers as a positive control (based on Abcam's testing) for the histone marks below. They may also be useful for other histone marks.

Suitable positive control for:
- Histone H3 tri methyl K27
- unmodified Histone H3
- Histone H3 mono methyl K4
- unmodified Histone H2B
- unmodified Histone H4
- Histone H3 mono methyl K9
- Histone H4 mono methyl K20
- unmodified Histone H2A

500pmole of each oligo per unit (lyophilised). HPLC purified, desalted and lyophilised as a sodium salt.

Quantity provided is sufficient for approx. 200 reactions based on 2.5pmol of primer per reaction with a final concentration of 100nM in 25μl.

Please contact us after purchase if you require the sequence of the oligos.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • ChIP - Myo-D positive control ChIP primer pair (ab269261), expandable thumbnail

    ChIP - Myo-D positive control ChIP primer pair (ab269261)

    Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade ab6002 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Myo-D positive control ChIP primer pair (ab269261), expandable thumbnail

    ChIP - Myo-D positive control ChIP primer pair (ab269261)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with 0.75% formaldehyde for 10min. The ChIP was performed with 25μg of chromatin, 2μg of Anti-Histone H3 (mono methyl K4) antibody [ERP16597] - ChIP Grade ab176877 (blue), and 20μl of Anti-rabbit IgG agarose beads. Rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the GAPDH and ALDOA (active) and MYO-D (inactive) promoters and over the γ-Actin gene (active). Schematic diagram of the γ-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

  • ChIP - Myo-D positive control ChIP primer pair (ab269261), expandable thumbnail

    ChIP - Myo-D positive control ChIP primer pair (ab269261)

    Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of Anti-Histone H3 (mono methyl K9) antibody [EPR16989] - ChIP Grade ab176880 (red), and 20μl of Protein A/G sepharose beads. Rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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