Phalloidin-iFluor 350 Reagent

Product details

Phalloidin-iFluor 350 Reagent (ab176751) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 350 can be easily detected with a fluorescent microscope at Ex/Em = 353/442 nm.

Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.

Each 300 test product includes 1 vial containing 30 µg of iFluor 350-dye-conjugated-phalloidin in 30 µl of DMSO at a concentration of 1 mg/mL. We recommend using this at a 1:1000 dilution, see protocol booklet for more instructions.

Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 (ab176753), Phalloidin-iFluor 647 (ab176759), Phalloidin-iFluor 594 (ab176757), Phalloidin-iFluor 555 (ab176756), and Rhodamine Phalloidin (ab235138), search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

Reagent Preparation

Before opening, briefly centrifuge the small vial at low speed to ensure all contents are collected at the bottom and to prevent loss of material.