Phalloidin-iFluor 405 Reagent ab176752 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin.
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- iScience 26:1071472023IFITM1 inhibits trophoblast invasion and is induced in placentas associated with IFN-mediated pregnancy diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSéverine A Degrelle,Julian Buchrieser,Anne Dupressoir,Françoise Porrot,Laurence Loeuillet,Olivier Schwartz,Thierry FournierPubMed 37434700
- Nature communications 14:16572023Temporal and spatial assembly of inner ear hair cell ankle link condensate through phase separation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuang Wang,Haibo Du,Rui Ren,Tingting Du,Lin Lin,Zhe Feng,Dange Zhao,Xiaoxi Wei,Xiaoyan Zhai,Hongyang Wang,Tingting Dong,Jin-Peng Sun,Hao Wu,Zhigang Xu,Qing LuPubMed 36964137
- Nature communications 14:12592023Mucociliary Wnt signaling promotes cilia biogenesis and beating.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarina Seidl,Fabio Da Silva,Kaiqing Zhang,Kai Wohlgemuth,Heymut Omran,Christof NiehrsPubMed 36878953
- Cell reports 42:1121432023Notch-dependent binary fate choice regulates the Netrin pathway to control axon guidance of Drosophila visual projection neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhang,Scott Lowe,Andrew Z Ding,Xin LiPubMed 36821442
- Cell reports 42:1121442023Axon targeting of Drosophila medulla projection neurons requires diffusible Netrin and is coordinated with neuroblast temporal patterning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhang,Scott Lowe,Andrew Z Ding,Xin LiPubMed 36821439
- eLife 12:2023Bilateral JNK activation is a hallmark of interface surveillance and promotes elimination of aberrant cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeepti Prasad,Katharina Illek,Friedericke Fischer,Katrin Holstein,Anne-Kathrin ClassenPubMed 36744859
- Cells 12:2023Actin-Cytoskeleton Drives Caveolae Signaling to Mitochondria during Postconditioning.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco Correa,Cristina Enríquez-Cortina,Alejandro Silva-Palacios,Nadia Román-Anguiano,Aurora Gil-Hernández,Marcos Ostolga-Chavarría,Elizabeth Soria-Castro,Sharik Hernández-Rizo,Paola de Los Heros,María Chávez-Canales,Cecilia ZazuetaPubMed 36766835
- Nature 614:144-1522022Programming multicellular assembly with synthetic cell adhesion molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam J Stevens,Andrew R Harris,Josiah Gerdts,Ki H Kim,Coralie Trentesaux,Jonathan T Ramirez,Wesley L McKeithan,Faranak Fattahi,Ophir D Klein,Daniel A Fletcher,Wendell A LimPubMed 36509107
- Cell death discovery 8:4592022RACK1 is evolutionary conserved in satellite stem cell activation and adult skeletal muscle regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisabetta Catalani,Silvia Zecchini,Matteo Giovarelli,Agnese Cherubini,Simona Del Quondam,Kashi Brunetti,Federica Silvestri,Paulina Roux-Biejat,Alessandra Napoli,Silvia Rosanna Casati,Marcello Ceci,Nicla Romano,Silvia Bongiorni,Giorgio Prantera,Emilio Clementi,Cristiana Perrotta,Clara De Palma,Davide CerviaPubMed 36396939
- Life science alliance 5:2022Measuring cystic fibrosis drug responses in organoids derived from 2D differentiated nasal epithelia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGimano D Amatngalim,Lisa W Rodenburg,Bente L Aalbers,Henriette Hm Raeven,Ellen M Aarts,Dounia Sarhane,Sacha Spelier,Juliet W Lefferts,Iris Al Silva,Wilco Nijenhuis,Sacha Vrendenbarg,Evelien Kruisselbrink,Jesse E Brunsveld,Cornelis M van Drunen,Sabine Michel,Karin M de Winter-de Groot,Harry G Heijerman,Lukas C Kapitein,Magarida D Amaral,Cornelis K van der Ent,Jeffrey M BeekmanPubMed 35922154
Key facts
- Target
- Beta-actin
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Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
Recommended products
Phalloidin-iFluor 405 Reagent ab176752 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin.
Key facts
- Target
- Beta-actin
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Phalloidin-iFluor 405 Reagent (ab176752) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 405 can be easily detected with a fluorescent microscope at Ex/Em = 400/421 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 (Phalloidin-iFluor 488 Reagent ab176753), Phalloidin-iFluor 647 (Phalloidin-iFluor 647 Reagent ab176759), Phalloidin-iFluor 594 (Phalloidin-iFluor 594 Reagent ab176757), Phalloidin-iFluor 555 (Phalloidin-iFluor 555 Reagent ab176756), and Rhodamine Phalloidin (Rhodamine Phalloidin Reagent ab235138), search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
F-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.
- Biological function summary
The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.
- Pathways
F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.
- Associated diseases and disorders
Abnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.
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2 product images
This image is courtesy of an anonymous customer review.Immunocytochemistry/ Immunofluorescence - Phalloidin-iFluor 405 Reagent (ab176752)
CytoPainter Phalloidin-iFluor 405 Reagent was used to stain F-actin in Bovine Foetal Aeorta Endothelial (BFA) cells. This was tested on triton x-100 permeabilised and non-permeabilised cells, phalloidin was prepared at 1 in 1000, diluted in 1% BSA in PBS. Cells were stained for 20 minutes. Coverslips with cells were then prepared and used for confocal microscopy. Nucleus was also stained with a nuclear stain that is excited by 633 laser. ctin staining worked in both permeabilised and unpermeabilised cells, although fluorescence was dimmer when compared to phalloidin-488 or phalloidin-568 stain, however this could be overcome perhaps by leaving the product on the cells for longer. Fluoresence also bleached relatively quickly, so it was important not to excite the fluorophore too strongly or for too long. Despite this, cells stained well, showing the same actin structures seen with other phalloidin stains and had the advantage in that the product could be used more dilute (1 in 1000) compared to other stains that are used at 1 in 25 dilution. I would recommend this product if 488 or 568 fluorophores could not be used.
Fluorescence Microscopy - Phalloidin-iFluor 405 Reagent (ab176752)
Excitation and emission spectra of phalloidin-iFluor 405 reagent
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