Phalloidin-iFluor 488 Reagent

Product details

Phalloidin-iFluor 488 Reagent ab176753 is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. The iFluor 488 dye can be easily detected with a fluorescent microscope at Ex/Em = 493/517 nm.

Each 300 test product includes 1 vial containing 30 µg of iFluor 488-dye-conjugated-phalloidin in 30 µl of DMSO at a concentration of 1 mg/mL. We recommend using this at a 1:1000 dilution, see protocol booklet for more instructions.

Our phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used with paraffin-embedded samples that have been de-paraffinized.

Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

Phalloidin protocol summary

• - Prepare samples in microplate wells
• - Remove liquid from samples in the plate
• - Add Phalloidin-iFluor™ 488 Conjugate solution (100 μL/well)
• - Stain the cells at room temperature for 20 to 90 minutes
• - Wash the cells
• - Examine the specimen under microscope with the appropriate filter

When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 (ab176753), Phalloidin-iFluor 647 (ab176759), Phalloidin-iFluor 555 (ab176756), and Rhodamine Phalloidin (ab235138), search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

Reagent Preparation

Before opening, briefly centrifuge the small vial at low speed to ensure all contents are collected at the bottom and to prevent loss of material.