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AB176755

Phalloidin-iFluor 532 Reagent

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(5 Publications)

Phalloidin-iFluor 532 Reagent (ab176755) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin.

View Alternative Names

Beta-actin, ACTB

1 Images
Fluorescence Microscopy - Phalloidin-iFluor 532 Reagent (AB176755)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Phalloidin-iFluor 532 Reagent (AB176755)

Excitation and emission spectra of phalloidin-iFluor 532 reagent

Key facts

Applications

Fluorescence Microscopy, ICC/IF

applications

Target

ACTB

target

Form

Liquid

form

Storage buffer

Constituents: 99% Dimethylsulfoxide, 1% Fluorescent-Phalloidin Conjugate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "Fluorescence Microscopy": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Phalloidin-iFluor 532 Reagent (ab176755) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 532 dye can be easily detected with a fluorescent microscope at Ex/Em = 542/558 nm.

Each 300 test product includes 1 vial containing 30 µg of iFluor 532-dye-conjugated-phalloidin in 30 µl of DMSO at a concentration of 1 mg/mL. We recommend using this at a 1:1000 dilution, see protocol booklet for more instructions.

Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.

Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.

Reagent Preparation

Before opening, briefly centrifuge the small vial at low speed to ensure all contents are collected at the bottom and to prevent loss of material.

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.
Biological function summary

The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.

Pathways

F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.

Abnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.
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Product protocols

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
See full target information ACTB
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Drug delivery and translational research : PubMed40478382

2025

Bisphosphonates loaded nanoparticles in microparticles: a potential macrophage targeting and repolarizing drug delivery system.

Applications

Unspecified application

Species

Unspecified reactive species

Paul N K Sagoe,Benjamin Zink,Era Jain

Nanomaterials (Basel, Switzerland) 14: PubMed38276736

2024

Pesticide Efficiency of Environment-Friendly Transition Metal-Doped Magnetite Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Shamaila Shahzadi,Jalees Ul Hassan,Muhammad Oneeb,Saira Riaz,Rehana Sharif,Dayan Ban

iScience 26:107305 PubMed37529103

2023

Short heat shock has a long-term effect on mesenchymal stem cells' transcriptome.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Ribarski-Chorev,Gisele Schudy,Carmit Strauss,Sharon Schlesinger

Biomicrofluidics 16:044101 PubMed35845724

2022

Long-term cultured microvascular networks on chip for tumor vascularization research and drug testing.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Zhang,Zhichang Du,Tianying Yuan,Jiajun Huang,Xiaoyu Zhao,Shengli Mi

Pharmaceutics 13: PubMed34834326

2021

Tropism of Extracellular Vesicles and Cell-Derived Nanovesicles to Normal and Cancer Cells: New Perspectives in Tumor-Targeted Nucleic Acid Delivery.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiya Oshchepkova,Oleg Markov,Evgeniy Evtushenko,Alexander Chernonosov,Elena Kiseleva,Ksenia Morozova,Vera Matveeva,Lyudmila Artemyeva,Valentin Vlassov,Marina Zenkova
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Product promise

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