Phalloidin-iFluor 594 Reagent

Product details

How the assay works

Phalloidin-iFluor 594 Reagent (equivalent to Alexa Fluor® 594-labeled phalloidin) selectively binds to F-actins. Phalloidin-Fluor 594 can be detected with a fluorescent microscope at Ex/Em = 590/618 nm.

Each 300 test product includes 1 vial containing 30 µg of iFluor 594-dye-conjugated-phalloidin in 30 µl of DMSO at a concentration of 1 mg/mL. We recommend using this at a 1:1000 dilution, see protocol booklet for more instructions.

Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized. Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

Phalloidin-iFluor 594 Reagent Protocol Summary

- Prepare samples in microplate wells
- Remove liquid from samples in the plate
- Add Phalloidin-iFluor 594 (100 µL/well)
- Stain the cells at room temperature for 20 to 90 minutes
- Wash the cells
- Examine the specimen under microscope with TRITC or Texas Red filter

How other researchers are using

Phalloidin-iFluor 594 Reagent has been used in a variety of sample type including:
- HeLa cells 1
- Breast cancer cell lines MDA-MB-231 and MCF-7 2
- Human liver cancer tissue 3

References:
1 - Juhlen R et al. 2024; 2 - Mombo B et al. 2023; 3 - Jagatia R et al. 2023.

Related and recommended products

Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 (ab176753), Phalloidin-iFluor 555 (ab176756) and Rhodamine Phalloidin (ab235138), search the website for all phalloidin conjugates, or read the phalloidin staining protocol.

Reagent Preparation

Before opening, briefly centrifuge the small vial at low speed to ensure all contents are collected at the bottom and to prevent loss of material.