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AB176758

Phalloidin-iFluor 633 Reagent

1

(1 Review)

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(13 Publications)

Phalloidin-iFluor 633 Reagent (ab176758) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin.

View Alternative Names

Beta-actin, ACTB

1 Images
Fluorescence Microscopy - Phalloidin-iFluor 633 Reagent (AB176758)
  • Fluorescence Microscopy

Supplier Data

Fluorescence Microscopy - Phalloidin-iFluor 633 Reagent (AB176758)

Excitation and emission spectra of phalloidin-iFluor 633 reagent.

Key facts

Applications

ICC/IF, Fluorescence Microscopy

applications

Target

ACTB

target

Form

Solid

form

Storage buffer

Constituents: 100% Fluorescent-Phalloidin Conjugate

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Reactivity data

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Product details

Phalloidin-iFluor 633 Reagent (ab176758) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 633 can be detected with a fluorescent microscope at Ex/Em = 634/649 nm.

Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.

Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 (ab176753), Phalloidin-iFluor 647 (ab176759), Phalloidin-iFluor 594 (ab176757), Phalloidin-iFluor 555 (ab176756), and Rhodamine Phalloidin (ab235138), search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.
Biological function summary

The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.

Pathways

F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.

Abnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.
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Product protocols

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Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
See full target information ACTB
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:8503 PubMed39424799

2024

De novo designed YK peptides forming reversible amyloid for synthetic protein condensates in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Miki,Masahiro Hashimoto,Hiroki Takahashi,Masatoshi Shimizu,Sae Nakayama,Tadaomi Furuta,Hisakazu Mihara

Nature communications 15:8377 PubMed39333081

2024

Metabolic regulation of cytoskeleton functions by HDAC6-catalyzed α-tubulin lactylation.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangshuang Sun,Zhe Xu,Liying He,Yihui Shen,Yuqing Yan,Xubing Lv,Xujing Zhu,Wei Li,Wei-Ya Tian,Yongjun Zheng,Sen Lin,Yadong Sun,Lei Li

Advanced healthcare materials 13:e2303928 PubMed38291861

2024

Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha G Zambuto,Ishita Jain,Hannah S Theriault,Gregory H Underhill,Brendan A C Harley

Frontiers in neuroscience 17:1162937 PubMed37144094

2023

Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A Lindner,Dennis Derstroff,Dominik Oliver,Katrin Reimann

Communications biology 6:92 PubMed36690695

2023

A high-throughput, 28-day, microfluidic model of gingival tissue inflammation and recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley L Gard,Rebeccah J Luu,Ryan Maloney,Madeline H Cooper,Brian P Cain,Hesham Azizgolshani,Brett C Isenberg,Jeffrey T Borenstein,Jane Ong,Joseph L Charest,Else M Vedula

ACS biomaterials science & engineering 8:3819-3830 PubMed35994527

2022

Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha G Zambuto,Ishita Jain,Kathryn B H Clancy,Gregory H Underhill,Brendan A C Harley

Frontiers in molecular neuroscience 14:754762 PubMed34975397

2021

Capping Protein Regulator and Myosin 1 Linker 3 (CARMIL3) as a Molecular Signature of Ischemic Neurons in the DWI-T2 Mismatch Areas After Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Joe Yeh,Pang-Hung Hsu,Ti-Yen Yeh,Wei-Kang Yang,Ko-Ping Chang,Chien-Sung Chiang,Sung-Chun Tang,Li-Kai Tsai,Jiann-Shing Jeng,Sung-Tsang Hsieh

Nature communications 12:3412 PubMed34099696

2021

Intracellular artificial supramolecules based on de novo designed Y15 peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Miki,Taichi Nakai,Masahiro Hashimoto,Keigo Kajiwara,Hiroshi Tsutsumi,Hisakazu Mihara

Biomedical optics express 12:1136-1153 PubMed33680563

2021

Three-dimensional characterization of collagen remodeling in cell-seeded collagen scaffolds via polarization second harmonic generation.

Applications

Unspecified application

Species

Unspecified reactive species

Dionysios Xydias,Georgios Ziakas,Sotiris Psilodimitrakopoulos,Andreas Lemonis,Eleni Bagli,Theodore Fotsis,Achille Gravanis,Dimitrios S Tzeranis,Emmanuel Stratakis

Cell 181:1596-1611.e27 PubMed32559461

2020

Metabolic Fingerprinting Links Oncogenic PIK3CA with Enhanced Arachidonic Acid-Derived Eicosanoids.

Applications

Unspecified application

Species

Unspecified reactive species

Nikos Koundouros,Evdoxia Karali,Aurelien Tripp,Adamo Valle,Paolo Inglese,Nicholas J S Perry,David J Magee,Sara Anjomani Virmouni,George A Elder,Adam L Tyson,Maria Luisa Dória,Antoinette van Weverwijk,Renata F Soares,Clare M Isacke,Jeremy K Nicholson,Robert C Glen,Zoltan Takats,George Poulogiannis
View all publications
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