Phalloidin-iFluor 680 Reagent

Product details

Phalloidin-iFluor 680 Reagent (ab176760) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phallodin-iFluor 680 can be detected with a fluorescent microscope at Ex/Em = 683/700 nm (NIR range).

Each 300 test product includes 1 vial containing 30 µg of iFluor 680-dye-conjugated-phalloidin in 30 µl of DMSO at a concentration of 1 mg/mL. We recommend using this at a 1:1000 dilution, see protocol booklet for more instructions.

Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.

Review our other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 ab176753, Phalloidin-iFluor 647, ab176759, Phalloidin-iFluor 594 ab176757, Phalloidin-iFluor 555 ab176756, and Rhodamine Phalloidin ab235138, or search our website to see the rest of the range.

Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

Reagent Preparation

Before opening, briefly centrifuge the small vial at low speed to ensure all contents are collected at the bottom and to prevent loss of material.