Phalloidin-iFluor 750 Reagent
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Phalloidin-iFluor 750 Reagent (ab176762) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin.
View Alternative Names
Beta-actin, ACTB
- Fluorescence Microscopy
Supplier Data
Fluorescence Microscopy - Phalloidin-iFluor 750 Reagent (AB176762)
Excitation and emission spectra of phalloidin-iFluor 750 reagent.
Complementary Products
Reactivity data
Product details
Phalloidin-iFluor 750 Reagent (ab176762) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 750 can be detected with a fluorescent microscope at Ex/Em = 752/778 nm (NIR range).
Each 300 test product includes 1 vial containing 30 µg of iFluor 750-dye-conjugated-phalloidin. Add in 30 µL of DMSO to make a concentration of 1 mg/mL. We recommend using this at a 1:1000 dilution, see protocol booklet for more instructions.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.
The vial containing ab176762 may appear to be empty. The packaging process involves aliquoting the compound dissolved in solvent and subsequent evaporation. This results in the deposition of a thin film of material that may not be clearly visible.
Reagent Preparation
Before opening, briefly centrifuge the small vial at low speed to ensure all contents are collected at the bottom and to prevent loss of material.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.
Pathways
F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.
Product protocols
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Target data
Product promise
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