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AB246832

Picro-Sirius Red Solution

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(40 Publications)

Picro-Sirius Red (PSR) Solution (ab246832) is suitable for histological visualization of muscle and collagen fibers, a major component of the extracellular matrix (ECM) in tissue sections in IHC. Suitable to quantify collagen content, fiber density, and organization.

- Used to differentiate collagens type I and type III fibers
- Suitable for light microscopy and polarized light microscopy
- Proven performance: cited in over 35 publications
- Compatible with our Lab essentials imaging workflow offering
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)

ab150681 (Picro Sirius Red stain) staining collagen (red), muscle fibres (yellow), cytoplasm (yellow) and red blood cells (yellow) in formalin fixed paraffin embedded normal human colon.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Picro-Sirius Red Solution (AB246832)

ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human skeletal muscle.

Key facts

Form

Liquid

form

Storage buffer

pH: 1 - 3 Constituents: 1.2% Picric Acid, 0.1% Direct Red 80

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Product details

Picro-Sirius Red Solution ab246832 is intended for use in the histological visualization of muscle and collagen fibers in tissue sections. The stain may be viewed using standard light microscopy or polarized light resulting in birefringence of collagen fibers. Please refer to ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) for more information on this solution as it is a component of the kit.

Light Microscopy
Collagen: Red
Muscle Fibers: Yellow
Cytoplasm: Yellow
Polarized Light Microscopy
Collagen: Yellow-Orange and Green Birefringence

Control Tissues: Lung, Uterus, Muscle, Kidney

Procedure:
1. Deparaffinize sections if necessary and hydrate to distilled water.
2. Apply adequate Picro-Sirius Red Solution to completely cover tissue section and incubate for 60 minutes.
3. Rinse slide quickly in two changes of Acetic Acid Solution (0.5%).
4. Rinse slide using absolute alcohol.
5. Dehydrate in 2 changes of absolute alcohol, clear, and mount in synthetic resin.

Other products for staining tissue sections

Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
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Product protocols

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Target data

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 58:e14402 PubMed40531753

2025

Protective effects of Aloe vera extract against doxorubicin-induced degeneration in ovarian follicles and stromal cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

E I T de Assis,A N Godinho,J M O Freire,M F de Lima Neto,J J N Costa,A L P Souza,A P O do Monte,M H T Matos,A L M de Sousa,J R V Silva,A W B Silva

Reproduction (Cambridge, England) 169: PubMed40344189

2025

Reproductive seasonality influences follicle dynamics and the ovarian extracellular matrix structural properties in ewes.

Applications

Unspecified application

Species

Unspecified reactive species

Johanne Grosbois,Vlastimil Srsen,Alba Muñoz Grande,Helen M Picton,Evelyn E Telfer

Redox biology 82:103554 PubMed40154102

2025

Redox regulation of lung endothelial PERK, unfolded protein response (UPR) and proliferation via NOX1: Targeted inhibition as a potential therapy for PAH.

Applications

Unspecified application

Species

Unspecified reactive species

Christian J Goossen,Alex Kufner,Christopher M Dustin,Imad Al Ghouleh,Shuai Yuan,Adam C Straub,John Sembrat,Jeffrey J Baust,Delphine Gomez,Damir Kračun,Patrick J Pagano

Genome biology 26:56 PubMed40087773

2025

A single-nucleus and spatial transcriptomic atlas of the COVID-19 liver reveals topological, functional, and regenerative organ disruption in patients.

Applications

Unspecified application

Species

Unspecified reactive species

Yered Pita-Juarez,Dimitra Karagkouni,Nikolaos Kalavros,Johannes C Melms,Sebastian Niezen,Toni M Delorey,Adam L Essene,Olga R Brook,Deepti Pant,Disha Skelton-Badlani,Pourya Naderi,Pinzhu Huang,Liuliu Pan,Tyler Hether,Tallulah S Andrews,Carly G K Ziegler,Jason Reeves,Andriy Myloserdnyy,Rachel Chen,Andy Nam,Stefan Phelan,Yan Liang,Mark Gregory,Shanshan He,Michael Patrick,Tushar Rane,Aster Wardhani,Amit Dipak Amin,Jana Biermann,Hanina Hibshoosh,Molly Veregge,Zachary Kramer,Christopher Jacobs,Yusuf Yalcin,Devan Phillips,Michal Slyper,Ayshwarya Subramanian,Orr Ashenberg,Zohar Bloom-Ackermann,Victoria M Tran,James Gomez,Alexander Sturm,Shuting Zhang,Stephen J Fleming,Sarah Warren,Joseph Beechem,Deborah Hung,Mehrtash Babadi,Robert F Padera,Sonya A MacParland,Gary D Bader,Nasser Imad,Isaac H Solomon,Eric Miller,Stefan Riedel,Caroline B M Porter,Alexandra-Chloé Villani,Linus T-Y Tsai,Winston Hide,Gyongyi Szabo,Jonathan Hecht,Orit Rozenblatt-Rosen,Alex K Shalek,Benjamin Izar,Aviv Regev,Yury V Popov,Z Gordon Jiang,Ioannis S Vlachos

Scientific reports 15:8685 PubMed40082498

2025

Expression of tumor endothelial marker 1 in cardiac fibroblast contributes to atrial fibrosis in atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Heng Li,Yu-Ning Hu,Ting-Chun Huang,Mu-Shiang Huang,Hsing-Chun Chung

Cell systems 16:101198 PubMed39970910

2025

Cold and hot fibrosis define clinically distinct cardiac pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Shoval Miyara,Miri Adler,Kfir B Umansky,Daniel Häußler,Elad Bassat,Yalin Divinsky,Jacob Elkahal,David Kain,Daria Lendengolts,Ricardo O Ramirez Flores,Hanna Bueno-Levy,Ofra Golani,Tali Shalit,Michael Gershovits,Eviatar Weizman,Alexander Genzelinakh,Danielle M Kimchi,Avraham Shakked,Lingling Zhang,Jingkui Wang,Andrea Baehr,Zachary Petrover,Rachel Sarig,Tatjana Dorn,Alessandra Moretti,Julio Saez-Rodriguez,Christian Kupatt,Elly M Tanaka,Ruslan Medzhitov,Achim Krüger,Avi Mayo,Uri Alon,Eldad Tzahor

Cancer research communications 5:195-208 PubMed39785683

2025

Targeting Pancreatic Cancer Cell Stemness by Blocking Fibronectin-Binding Integrins on Cancer-Associated Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Chengsheng Wu,Tami Von Schalscha,Diva Sansanwal,Chen Qian,Qinlin Jiang,Ryan M Shepard,Hiromi I Wettersten,Stephen J McCormack,Sara M Weis,David A Cheresh

Advanced healthcare materials 13:e2401480 PubMed39388502

2024

Accelerating Oral Wound Healing Using Bilayer Biomaterial Delivery of FTY720 Immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Afra I Toma,Daniel Shah,Daniela Roth,Jeremie Oliver Piña,Lauren Hymel,Thomas Turner,Archana Kamalakar,Ken Liu,Perry Bartsch,Leon Jacobs,Rena D'Souza,Dennis Liotta,Edward Botchwey,Nick J Willett,Steven L Goudy

Nature communications 15:8519 PubMed39353976

2024

CHIP ameliorates nonalcoholic fatty liver disease via promoting K63- and K27-linked STX17 ubiquitination to facilitate autophagosome-lysosome fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunjin Rho,Seungyeon Kim,Seung Up Kim,Jeong Won Kim,Sang Hoon Lee,Sang Hoon Park,Freddy E Escorcia,Joon-Yong Chung,Jaewhan Song

Nutrients 16: PubMed39125400

2024

Promoting Glutathione Synthesis: A Possibility for Treating Cardiomyopathy Induced by a Maternal Western Diet.

Applications

Unspecified application

Species

Unspecified reactive species

Jialing Zhang,Jiayu Wang,Da Xu,Yiting Gui,Fan Bai,Yu Huo,Li Cao,Yonghao Gui
View all publications
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