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Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes.


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SDS-PAGE - Ponceau S Solution (AB270042), expandable thumbnail

Key facts

Form
Liquid

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Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes.

Key facts

Form
Liquid
Storage buffer

Constituents: 0.96% Acetic acid, 0.1% Ponceau S

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

Notes

Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes. As little as 250 ng protein can be rapidly visualized with Ponceau S stain. Following staining, membranes can be de-stained with any buffer of pH >7 or by transfer of the membrane into blocking buffer (e.g. TBS/BSA).

This product is manufactured by Expedeon, an Abcam company. It was previously called Ponceau S Solution - 1L. Expedeon product code 5999-1L is the same as the 1000 mL size of this product.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • SDS-PAGE - Ponceau S Solution (ab270042), expandable thumbnail

    SDS-PAGE - Ponceau S Solution (ab270042)

    Runblue 4-12% Bis-Tris gel.

    Lanes 1 to 8 Thermo Scientific Page Ruler Plus, lane 2 E.coli lysate, lane 3 Chicken lysate, lane 4 2 μg BSA, lane 5 1 μg BSA, lane 6 500 ng BSA, lane 7 250 ng BSA.

    Following membrane transfer, users can directly incubate the blotting membrane (PVDF or nitrocellulose) in ~15ml of Ponceau S solution without the need for any wash steps. All transferred protein slowly becomes visible as red bands on the membrane, with highest intensity of staining reached within 5 minutes incubation with Ponceau S. Rinsing the membrane with water after Ponceau S staining can help reduce 'background' staining on the blotting membrane. The intensity of staining corresponds to the amount of protein transferred.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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