Ponceau S Solution
4
(1 Review)
|
(10 Publications)
- Provided as a ready-to-use 1X solution
- Suitable for both proteins run under both reducing and non-reducing conditions
- Suitable with various blocking agents including BSA, non-fat dry milk, casein and fish skin gelatin
- SDS-PAGE
Supplier Data
SDS-PAGE - Ponceau S Solution (AB270042)
Runblue 4-12% Bis-Tris gel.
Lanes 1 to 8 Thermo Scientific Page Ruler Plus, lane 2 E.coli lysate, lane 3 Chicken lysate, lane 4 2 μg BSA, lane 5 1 μg BSA, lane 6 500 ng BSA, lane 7 250 ng BSA.
Following membrane transfer, users can directly incubate the blotting membrane (PVDF or nitrocellulose) in ~15ml of Ponceau S solution without the need for any wash steps. All transferred protein slowly becomes visible as red bands on the membrane, with highest intensity of staining reached within 5 minutes incubation with Ponceau S. Rinsing the membrane with water after Ponceau S staining can help reduce 'background' staining on the blotting membrane. The intensity of staining corresponds to the amount of protein transferred.
Product details
Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes. As little as 250 ng protein can be rapidly visualized with Ponceau S stain. Following staining, membranes can be de-stained with any buffer of pH >7 or by transfer of the membrane into blocking buffer (e.g. TBS/BSA).
This product is manufactured by Expedeon, an Abcam company. It was previously called Ponceau S Solution - 1L. Expedeon product code 5999-1L is the same as the 1000 mL size of this product.
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Publications (10)
Recent publications for all applications. Explore the full list and refine your search
The Journal of experimental medicine 220: PubMed37642941
2023
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in cellular neuroscience 17:1170031 PubMed37234916
2023
Applications
Unspecified application
Species
Unspecified reactive species
Methods and protocols 6: PubMed36961042
2023
Applications
Unspecified application
Species
Unspecified reactive species
Journal of Alzheimer's disease : JAD 92:1027-1035 PubMed36847010
2023
Applications
Unspecified application
Species
Unspecified reactive species
American journal of physiology. Cell physiology 324:C339-C352 PubMed36440857
2022
Applications
Unspecified application
Species
Unspecified reactive species
Nature 608:609-617 PubMed35948633
2022
Applications
Unspecified application
Species
Unspecified reactive species
Current protocols 2:e497 PubMed35849593
2022
Applications
Unspecified application
Species
Unspecified reactive species
STAR protocols 3:101449 PubMed35693212
2022
Applications
Unspecified application
Species
Unspecified reactive species
Chemico-biological interactions 356:109882 PubMed35263611
2022
Applications
Unspecified application
Species
Unspecified reactive species
Current protocols 1:e277 PubMed34748287
2021
Applications
Unspecified application
Species
Unspecified reactive species
Complementary Products
Reagents, Controls & Accessories
AB116027
Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa)
- 5
- 2
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com