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Propidium Iodide (PI) provided in a convenient solution for staining double-stranded DNA.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
- Applications
Fluorescence Microscopy, Flow Cyt
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Fluorescence Microscopy | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
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Propidium Iodide (PI) provided in a convenient solution for staining double-stranded DNA.
Is this your first time purchasing this assay kit? Get 20% off one kit by entering code 20OFF-T5A8Q at checkout. T&C’s apply – click here. Offer only valid when purchasing directly from Abcam in Europe, North America, Australia, and Singapore.
Key facts
- Applications
Fluorescence Microscopy, Flow Cyt
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.
For Microscopy analysis: PI can be viewed using rhodamine(red) filter (λ= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).
For Flow Cytometry analysis: PI staining can be monitored in FL2 channel.
If used together as control for Annexin V assays ab14082, ab14083 or ab14152, PI should be diluted to 250 μg/ml solution (in PBS) prior use and added as 1 μl/ Annexin V Assay (0.25μg/assay).
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called 1056 Propidium Iodide. 1056-1 is the same size as the 1 ml size of ab14083.
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We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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- Visit the general protocols
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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