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Propidium Iodide (PI) ab14083 is provided in a convenient solution for staining double-stranded DNA.

Propidium Iodide (PI) ab14083 is a fluorescent DNA stain that specifically binds to double-stranded DNA where the plasma membrane has been compromised/ permeabilized. It does not penetrate live cells.
- Use in fluorescence microscopy and flow cytometry
- Cited in over 50 publications.

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Images

Immunofluorescence - Propidium Iodide (AB14083), expandable thumbnail
  • Flow Cytometry - Propidium Iodide (AB14083), expandable thumbnail

Publications

Key facts

Applications
Fluorescence Microscopy, Flow Cyt
Form
Liquid

Reactivity data

Application
Fluorescence Microscopy
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Recommended products

Propidium Iodide (PI) ab14083 is provided in a convenient solution for staining double-stranded DNA.

Propidium Iodide (PI) ab14083 is a fluorescent DNA stain that specifically binds to double-stranded DNA where the plasma membrane has been compromised/ permeabilized. It does not penetrate live cells.
- Use in fluorescence microscopy and flow cytometry
- Cited in over 50 publications.

Key facts

Applications
Fluorescence Microscopy, Flow Cyt
Form
Liquid
Storage buffer

pH: 7.4
Constituents: 99.9% PBS, 0.1% Propidium Iodide

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.

For Microscopy analysis: PI can be viewed using rhodamine(red) filter (λ= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).

For Flow Cytometry analysis: PI staining can be monitored in FL2 channel.

If used together as control for Annexin V assays ab14082, ab14083 or ab14152, PI should be diluted to 250 μg/ml solution (in PBS) prior use and added as 1 μl/ Annexin V Assay (0.25μg/assay).

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called 1056 Propidium Iodide. 1056-1 is the same size as the 1 ml size of ab14083.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Immunofluorescence - Propidium Iodide (ab14083), expandable thumbnail
    Image from Melia MM et al ., Plos One.,29 (8). Fig 1b DOI: 10.1371/journal.pone.0106281. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunofluorescence - Propidium Iodide (ab14083)

    Vero-DogSLAM (VDS) cells and Vero (African Green Monkey kidney) cells were infected with wtCDV. Cells were fixed, permeabilised and stained with SSPE serum and rabbit anti-human FITC; nuclei were stained using ab14083 propidium iodide. Images were taken using a Nikon Eclipse TE2000-U UV microscope (x400).

  • Flow Cytometry - Propidium Iodide (ab14083), expandable thumbnail
    Image from Wang et al., PLoS One., 10(9). Fig 3a & c. doi:10.1371/journal.pone.0137712 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Flow Cytometry - Propidium Iodide (ab14083)

    Wang et al. used ab14083 to investigate an optimized platform for maintaining proliferation of giant panda mesenchymal stem cells (MSCs). MSCs were fixed in 70% alcohol and 30% PBS at 4°C for 1 hour. Cells are then incubated in the dark with PBS, 20μg/ml propidium iodide (ab14083) and 1% RNaseA for 30 minutes at 37°C. Cell samples are resuspended in PBS and analyzed using FACS Calibur flow cytometry.

    Flow cytometry analysis shows the cells are at different cell cycle stages in different concentrations (0ng/ml and 5ng/ml respectively) of basic fibroblast growth factor (bFGF).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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