Propidium Iodide (PI) ab14083 is provided in a convenient solution for staining double-stranded DNA.
Propidium Iodide (PI) ab14083 is a fluorescent DNA stain that specifically binds to double-stranded DNA where the plasma membrane has been compromised/ permeabilized. It does not penetrate live cells.
- Use in fluorescence microscopy and flow cytometry
- Cited in over 50 publications.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application Fluorescence Microscopy | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Propidium Iodide (PI) ab14083 is provided in a convenient solution for staining double-stranded DNA.
Propidium Iodide (PI) ab14083 is a fluorescent DNA stain that specifically binds to double-stranded DNA where the plasma membrane has been compromised/ permeabilized. It does not penetrate live cells.
- Use in fluorescence microscopy and flow cytometry
- Cited in over 50 publications.
pH: 7.4
Constituents: 99.9% PBS, 0.1% Propidium Iodide
Propidium Iodide (PI) binds to double-stranded DNA. PI cannot cross intact plasma membrane and therefore will only be present in DNA of cells where the plasma membrane has been compromised/ permeabilized.
For Microscopy analysis: PI can be viewed using rhodamine(red) filter (λ= 536/617). Cells will only be stained if the membrane has been permeated, either naturally (non-viable cells) or with detergents (for fluorescent staining).
For Flow Cytometry analysis: PI staining can be monitored in FL2 channel.
If used together as control for Annexin V assays ab14082, ab14083 or ab14152, PI should be diluted to 250 μg/ml solution (in PBS) prior use and added as 1 μl/ Annexin V Assay (0.25μg/assay).
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This product is manufactured by BioVision, an Abcam company and was previously called 1056 Propidium Iodide. 1056-1 is the same size as the 1 ml size of ab14083.
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Vero-DogSLAM (VDS) cells and Vero (African Green Monkey kidney) cells were infected with wtCDV. Cells were fixed, permeabilised and stained with SSPE serum and rabbit anti-human FITC; nuclei were stained using ab14083 propidium iodide. Images were taken using a Nikon Eclipse TE2000-U UV microscope (x400).
Wang et al. used ab14083 to investigate an optimized platform for maintaining proliferation of giant panda mesenchymal stem cells (MSCs). MSCs were fixed in 70% alcohol and 30% PBS at 4°C for 1 hour. Cells are then incubated in the dark with PBS, 20μg/ml propidium iodide (ab14083) and 1% RNaseA for 30 minutes at 37°C. Cell samples are resuspended in PBS and analyzed using FACS Calibur flow cytometry.
Flow cytometry analysis shows the cells are at different cell cycle stages in different concentrations (0ng/ml and 5ng/ml respectively) of basic fibroblast growth factor (bFGF).
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