Protein blocking buffer ab64226 for serum-free blocking of non-specific antibody binding in IHC, ELISA and western blot.
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- Biomolecules 14:2024Evaluation of the Mechanisms Involved in the Development of Bladder Toxicity following Exposure to Occupational Bladder Cancer Causative Chemicals Using DNA Adductome Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShugo Suzuki,Min Gi,Masami Komiya,Asuka Obikane,Arpamas Vachiraarunwong,Masaki Fujioka,Anna Kakehashi,Yukari Totsuka,Hideki WanibuchiPubMed 38254636
- Frontiers in immunology 14:12825772023Identification of novel immune cell signature in gastroesophageal reflux disease: altered mucosal mast cells and dendritic cell profile.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhsen Ustaoglu,Fatema Arif Daudali,Manfredi D'afflitto,Stephen Murtough,Chung Lee,Estefania Moreno,Diana C Blaydon,David P Kelsell,Daniel Sifrim,Philip Woodland,Madusha PeirisPubMed 38098488
- Scientific reports 13:200192023Transthyretin amyloid deposition in ligamentum flavum (LF) is significantly correlated with LF and epidural fat hypertrophy in patients with lumbar spinal stenosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazuya Maeda,Kazuki Sugimoto,Masayoshi Tasaki,Takuya Taniwaki,Takahiro Arima,Yuto Shibata,Makoto Tateyama,Tatsuki Karasugi,Takanao Sueyoshi,Tetsuro Masuda,Yusuke Uehara,Takuya Tokunaga,Satoshi Hisanaga,Masaki Yugami,Ryuji Yonemitsu,Katsumasa Ideo,Kozo Matsushita,Yuko Fukuma,Masaru Uragami,Junki Kawakami,Naoto Yoshimura,Kosei Takata,Masaki Shimada,Shuntaro Tanimura,Hideto Matsunaga,Yuki Kai,Shu Takata,Ryuta Kubo,Rui Tajiri,Fuka Homma,Xiao Tian,Mitsuharu Ueda,Takayuki Nakamura,Takeshi MiyamotoPubMed 37973808
- Bone & joint research 12:615-6232023Regeneration of injured articular cartilage using the recombinant human amelogenin protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmer Helwa-Shalom,Faris Saba,Elad Spitzer,Salem Hanhan,Koby Goren,Shany I Markowitz,Dekel Shilo,Nissim Khaimov,Yechiel N Gellman,Dan Deutsch,Anat Blumenfeld,Hani Nevo,Amir HazePubMed 37783468
- Biomedicines 11:2023Connexin 37, 40, 43 and Pannexin 1 Expression in the Gastric Mucosa of Patients with Systemic Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBerna Pavic,Marin Ogorevc,Katarina Boric,Dubravka Vukovic,Mirna Saraga-Babic,Snjezana MardesicPubMed 37760928
- EJNMMI research 13:672023Synthesis and preclinical evaluation of a novel fluorine-18 labeled small-molecule PET radiotracer for imaging of CXCR3 receptor in mouse models of atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSantosh R Alluri,Yusuke Higashi,Ashley Berendzen,Laurel A Grisanti,Lisa D Watkinson,Kamlendra Singh,Timothy J Hoffman,Terry Carmack,Elizabeth A Devanny,Miles Tanner,Kun-Eek KilPubMed 37438543
- Bone research 11:342023A novel multifunctional radioprotective strategy using P7C3 as a countermeasure against ionizing radiation-induced bone loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFei Wei,Zewen Kelvin Tuong,Mahmoud Omer,Christopher Ngo,Jackson Asiatico,Michael Kinzel,Abinaya Sindu Pugazhendhi,Annette R Khaled,Ranajay Ghosh,Melanie CoathupPubMed 37385982
- Journal of clinical biochemistry and nutrition 73:191-1972023Induction of -amplified neuroblastoma differentiation through NMYC suppression using PPAR-γ antagonist.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYukako Nakao-Ise,Takumi Narita,Shingo Miyamoto,Motoki Watanabe,Takuji Tanaka,Yoshihiro Sowa,Yosuke Iizumi,Mitsuharu Masuda,Gen Fujii,Yasuko Hirai,Toshimasa Nakao,Hideki Takakura,Michihiro MutohPubMed 37970556
- International journal of molecular sciences 24:2023The Effect of GLUT1 and HIF-1α Expressions on Glucose Uptake and Patient Survival in Non-Small-Cell Lung Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosipa Kokeza,Ante Strikic,Marin Ogorevc,Nela Kelam,Martina Vukoja,Ivo Dilber,Sandra Zekic TomasPubMed 37445752
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 71:301-3202023Developmental Expression of the Cell Cycle Regulator p16 in Retinal Glial Cells: A Novel Marker for Immature Ocular Astrocytes?Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Martinez-Fernandez de la Camara,Tina Storm,Ahmed Salman,Thomas Burgoyne,Martin Qvist Rasmussen,Harry O Orlans,Angela J Russell,Stephen G Davies,Alun R Barnard,Robert E MacLarenPubMed 37350564
Key facts
- Applications
ELISA, IHC-P, WB
- Form
Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Protein blocking buffer ab64226 for serum-free blocking of non-specific antibody binding in IHC, ELISA and western blot.
Key facts
- Applications
ELISA, IHC-P, WB
- Form
Liquid
- Storage buffer
pH: 7.2 - 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 0.5% BSA, 0.5% Casein
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Protein Block has been developed to use with immunolabeling techniques for the reduction of nonspecific background staining while simultaneously reducing the handling of animal serums in the laboratory. The need to match species with the secondary antibody is eliminated due to the lack of normal serum in this product. Protein Block has been shown to be effective for immunohistochemical, ELISA, and blot and requires no mixing or diluting.
Protein blocking buffer ab64226 for serum-free blocking of non-specific antibody binding in IHC, ELISA and western blot. Ready to use. No mixing or dilution required.
For use in ELISA, add Protein Block to well and incubate for 2-10 minutes before addition of sample. Wash and continue procedure. For use in western blotting, incubate for one hour at room temperature or overnight at 4°C.
IHC protocol suitable for use with Protein Block ab64226:
For frozen sections, skip steps 1 and 2. For fluorescent IHC, skip step 3, incubate with fluorescent dye conjugated secondary at step 6, skip rest of steps, and mount with anti-fade mounting medium.
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply Protein Block ab64226 (or normal serum from same species as secondary antibody) and incubate for at least 30 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in antibody diluent and incubate. Wash 4 times in buffer.
6. Incubate with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC substrate and incubate until desired color is achieved. Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
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6 product images
Immunocytochemistry - Protein Block (ab64226)
NUCB1/NLP and NUCB2/NESF colocalizes with GH in somatotrophsRepresentative images of immunofluorescence detection of NUCB1 (green), NUCB2 (green) and GH (red) in GH3 cells. Cells were blocked with an antibody blocking buffer (ABB) based in PBS consisting of 3% BSA, 0.05% Triton X-100 and 10% of protein block solution (ab64226). Cells were counterstained with DAPI (blue) and the images were acquired at 40X magnification.
Flow Cytometry - Protein Block (ab64226)
GLUT2 expression in beta cells of islets after treatment with necrostatin-1.islet were dissociated into single cells, fixed and permeabilised before incubation with Protein Block (ab64226) on ice for 30 minutes to decrease non-specific binding. Then cells were labelled using a FITC-conjugated anti-GLUT2.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein Block (ab64226)
Immunofluorescence staining of MPO and CD68 in rat heart(A,B) MPO (Anti-Myeloperoxidase antibody ab9535, diluted at 1:100) immunoreactivity on a cross section of the heart showing the transmural ischemic zone (IZ) of the anterior wall of the left ventricle with subepicardial (PIZ-EPI) and peripheral (PIZ-1) peri-infarct zones; (C) MPO- (red, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150084, diluted 1:200) and CD68 (Anti-CD68 antibody [ED1] ab31630, diluted at 1:250)-immunoreactivity (green, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, dilued 1:200) of the peri-infarct zone showing no co-localisation of fluorescence; (D) MPO-immunoreactivity is found in the cytoplasm of polymorphonuclear cells (white arrows); (E) a layer of cellular profiles, immunoreactive for both the MPO and CD68 detected within the subepicardial myocardium; (F) CD68-immunoreactivity is found in the cytoplasm of mononuclear cells (white arrows). Scale bars: A = 0.5 mm, B = 200 ?m, C = 75 ?m, D,F = 20 ?m, E = 50 ?m.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein Block (ab64226)
Light micrographs of cerebellar sections with immunohistochemistry by anti-caspase 3 antibodies.Adult male albino rat demonstrating mild caspase-3 immunoreactivity in the three cerebellar layers. Incubation of sections was done with rabbit monoclonal caspase-3 antibodies (Abcam, Cambridge, UK), 1:200 dilutions for 1?h.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein Block (ab64226)
ab64226 was used as pasr of the Mouse and Rabbit Specific HRP Detection IHC Kit ab93677 anti-Mouse and Rabbit specific HRP Detection IHC kit to perform immunohistochemical analysis of Human Tonsil tissue labeling Ki-67.
Yu S.H., PLoS One 9 (12), Fig 7c. doi: 10.1371/journal.pone.0114649 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein Block (ab64226)
ab64226 was used in the mouse and rabbit specific HRP/DAB detection IHC Kit (Mouse and Rabbit Specific HRP/DAB Detection IHC kit ab64264) to visualize Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution.
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