Protein Block

• ICC

PubMed

Immunocytochemistry - Protein Block (AB64226)

NUCB1/NLP and NUCB2/NESF colocalizes with GH in somatotrophsRepresentative images of immunofluorescence detection of NUCB1 (green), NUCB2 (green) and GH (red) in GH3 cells. Cells were blocked with an antibody blocking buffer (ABB) based in PBS consisting of 3% BSA, 0.05% Triton X-100 and 10% of protein block solution (ab64226). Cells were counterstained with DAPI (blue) and the images were acquired at 40X magnification.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein Block (AB64226)

Immunofluorescence staining of MPO and CD68 in rat heart(A,B) MPO (ab9535, diluted at 1 : 100) immunoreactivity on a cross section of the heart showing the transmural ischemic zone (IZ) of the anterior wall of the left ventricle with subepicardial (PIZ-EPI) and peripheral (PIZ-1) peri-infarct zones; (C) MPO- (red, ab150084, diluted 1 : 200) and CD68 (ab31630, diluted at 1 : 250)-immunoreactivity (green, ab150117, diluted 1 : 200) of the peri-infarct zone showing no co-localisation of fluorescence; (D) MPO-immunoreactivity is found in the cytoplasm of polymorphonuclear cells (white arrows); (E) a layer of cellular profiles, immunoreactive for both the MPO and CD68 detected within the subepicardial myocardium; (F) CD68-immunoreactivity is found in the cytoplasm of mononuclear cells (white arrows). Scale bars : A = 0.5 mm, B = 200 µm, C = 75 µm, D,F = 20 µm, E = 50 µm.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein Block (AB64226)

Light micrographs of cerebellar sections with immunohistochemistry by anti-caspase 3 antibodies.Adult male albino rat demonstrating mild caspase-3 immunoreactivity in the three cerebellar layers. Incubation of sections was done with rabbit monoclonal caspase-3 antibodies (Abcam, Cambridge, UK), 1 : 200 dilutions for 1 h.

• Flow Cyt

PubMed

Flow Cytometry - Protein Block (AB64226)

GLUT2 expression in beta cells of islets after treatment with necrostatin-1.islet were dissociated into single cells, fixed and permeabilised before incubation with Protein Block (ab64226) on ice for 30 minutes to decrease non-specific binding. Then cells were labelled using a FITC-conjugated anti-GLUT2.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein Block (AB64226)

ab64226 was used as pasr of the ab93677 anti-Mouse and Rabbit specific HRP Detection IHC kit to perform immunohistochemical analysis of Human Tonsil tissue labeling Ki-67.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Protein Block (AB64226)

ab64226 was used in the mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264) to visualize Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1 : 450 dilution.

Yu S.H., PLoS One 9 (12), Fig 7c. doi: 10.1371/journal.pone.0114649 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/