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Proteinase K antigen retrieval solution is a ready-to-use, single-component solution designed for proteolytic-induced antigen retrieval (PIER) in immunohistochemistry (IHC) and pre-treatment for in situ hybridization (ISH).


- Cited in over 25 publications
- Ready-to-Use – no preparation needed for immediate application
- Incubate tissue section for 5-7 minutes at room temperature in IHC
- Incubate tissue section for 15 minutes at 37°C in ISH

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K Antigen Retrieval Solution (AB64220), expandable thumbnail

Publications

Key facts

Applications
IHC-P, ISH
Form
Liquid

Reactivity data

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ISH
Reactivity
Reacts
Dilution info
-
Notes

-

Recommended products

Proteinase K antigen retrieval solution is a ready-to-use, single-component solution designed for proteolytic-induced antigen retrieval (PIER) in immunohistochemistry (IHC) and pre-treatment for in situ hybridization (ISH).


- Cited in over 25 publications
- Ready-to-Use – no preparation needed for immediate application
- Incubate tissue section for 5-7 minutes at room temperature in IHC
- Incubate tissue section for 15 minutes at 37°C in ISH

Key facts

Applications
IHC-P, ISH
Form
Liquid
Storage buffer

pH: 7 - 7.4
Preservative: 0.1% Sodium azide
Constituents: Tris buffer, 10% Glycerol (glycerin, glycerine)

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

ab64220 is a single-component, ready-to-use proteinase K solution for use in protease-induced antigen retrieval during IHC (PIER) and pre-treatment for in situ hybridization.

Staining Protocol:

  1. IHC procedure: Incubate tissue section for 5 to 7 minutes at room temperature; Rinse and proceed with immunostaining.
  2. General ISH procedure: Incubate tissue section for 15 minutes at 37oC; Rinse and proceed with hybridization.

Other products for IHC

Other enzymes for antigen retrieval include: Pepsin Solution ab64201 and Trypsin Antigen Retrieval Kit ab259256.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K Antigen Retrieval Solution (ab64220), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K Antigen Retrieval Solution (ab64220)

    ab64220 Proteinase K used with aa anti-Pan CK antibody on Human skin

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com