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AB64220

Proteinase K Antigen Retrieval Solution

5

(1 Review)

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(36 Publications)

Proteinase K antigen retrieval solution (ab64220) is a ready-to-use, single-component solution designed for proteolytic-induced antigen retrieval (PIER) in immunohistochemistry (IHC) and pre-treatment for in situ hybridization (ISH).

- Proven performance: cited in over 35 publications
- Compatible with our Lab essentials imaging workflow offering.
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K Antigen Retrieval Solution (AB64220)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K Antigen Retrieval Solution (AB64220)

ab64220 Proteinase K used with aa anti-Pan CK antibody on Human skin

Key facts

Applications

IHC-P, ISH

applications

Form

Liquid

form

Storage buffer

pH: 7 - 7.4 Preservative: 0.1% Sodium azide Constituents: Tris buffer, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ISH": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab64220 is a single-component, ready-to-use proteinase K solution for use in protease-induced antigen retrieval during IHC (PIER) and pre-treatment for in situ hybridization.

Staining Protocol:

  1. IHC procedure: Incubate tissue section for 5 to 7 minutes at room temperature; Rinse and proceed with immunostaining.
  2. General ISH procedure: Incubate tissue section for 15 minutes at 37oC; Rinse and proceed with hybridization.

Other products for IHC

Other enzymes for antigen retrieval include: Pepsin Solution ab64201 and Trypsin Antigen Retrieval Kit ab259256.

Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

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Target data

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1595319 PubMed40607410

2025

Evaluating the immunogenicity of a mouse partial hindlimb for composite allotransplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Hui Angela Sun,Samantha Ligi,Felor Biniazan,Siba Haykal

International journal of molecular sciences 26: PubMed40244257

2025

The Effects of the Natriuretic Peptide System on Alveolar Epithelium in Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Yara Knany,Safa Kinaneh,Emad E Khoury,Yaniv Zohar,Zaid Abassi,Zaher S Azzam

Oncology letters 29:196 PubMed40046638

2025

Profile and clinical significance of SPARCL1 and its prognostic significance in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yu Xu,Ying-Wen Han,Yun-Xiao Song,Zhen-Yu Zhou,Shu Chen,Yu-Wei Liu,Ying Zhou,Jie Chen

Arthritis research & therapy 27:40 PubMed40001226

2025

Targeting osteoclast-derived DPP4 alleviates inflammation-mediated ectopic bone formation in ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

Seung Hoon Lee,Kyu Hoon Lee,Dongju Kim,Chanhyeok Jeon,Min Whangbo,Hye-Ryeong Jo,Jeehee Youn,Chang-Hun Lee,Sung Hoon Choi,Ye-Soo Park,Bora Nam,Sungsin Jo,Tae-Hwan Kim

Frontiers in immunology 15:1469424 PubMed39450167

2024

Intranasal sensitization model of alopecia areata using pertussis toxin as adjuvant.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Liu,Jasmin Freeborn,Beanna Okeugo,Shabba A Armbrister,Zeina M Saleh,Ana Beatriz Fadhel Alvarez,Thomas K Hoang,Evelyn S Park,John William Lindsey,Ronald P Rapini,Steven Glazer,Keith Rubin,Jon Marc Rhoads

Journal of cellular and molecular medicine 28:e18512 PubMed39248454

2024

Involvement of heparanase in the pathogenesis of acute pancreatitis: Implication of novel therapeutic approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Dalit B Hamo-Giladi,Ahmad Fokra,Edmond Sabo,Aviva Kabala,Irena Minkov,Shadi Hamoud,Salim Hadad,Zaid Abassi,Iyad Khamaysi

Acta pharmaceutica Sinica. B 14:2057-2076 PubMed38799632

2024

The raphe nuclei are the early lesion site of gastric -synuclein propagation to the substantia nigra.

Applications

Unspecified application

Species

Unspecified reactive species

Chenglu Zhang,Ruxue Bo,Tiantian Zhou,Naihong Chen,Yuhe Yuan

Arthritis research & therapy 26:2 PubMed38167497

2024

Exosome-mediated delivery of super-repressor IκBα alleviates inflammation and joint damages in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Hae-In Lee,Min-Joo Ahn,Jae-Kwang Yoo,So-Hee Ahn,Seon Young Park,Hyangmi Seo,Moon-Ju Kim,Yu Jeong Lee,Hyun Hee Jang,Seung Cheol Shim,Eun Jeong Won,Cheolhyoung Park,Chulhee Choi,Tae-Jong Kim

Neuro-oncology 26:609-622 PubMed37767814

2023

Intraventricular SHH inhibition proves efficient in SHH medulloblastoma mouse model and prevents systemic side effects.

Applications

Unspecified application

Species

Unspecified reactive species

Catena Kresbach,Lea Holst,Melanie Schoof,Tara Leven,Carolin Göbel,Sina Neyazi,Jacqueline Tischendorf,Carolin Loose,Antonina Wrzeszcz,Timur Yorgan,Stefan Rutkowski,Ulrich Schüller

Nature 619:801-810 PubMed37438528

2023

Spatially resolved multiomics of human cardiac niches.

Applications

Unspecified application

Species

Unspecified reactive species

Kazumasa Kanemaru,James Cranley,Daniele Muraro,Antonio M A Miranda,Siew Yen Ho,Anna Wilbrey-Clark,Jan Patrick Pett,Krzysztof Polanski,Laura Richardson,Monika Litvinukova,Natsuhiko Kumasaka,Yue Qin,Zuzanna Jablonska,Claudia I Semprich,Lukas Mach,Monika Dabrowska,Nathan Richoz,Liam Bolt,Lira Mamanova,Rakeshlal Kapuge,Sam N Barnett,Shani Perera,Carlos Talavera-López,Ilaria Mulas,Krishnaa T Mahbubani,Liz Tuck,Lu Wang,Margaret M Huang,Martin Prete,Sophie Pritchard,John Dark,Kourosh Saeb-Parsy,Minal Patel,Menna R Clatworthy,Norbert Hübner,Rasheda A Chowdhury,Michela Noseda,Sarah A Teichmann
View all publications
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Complementary Products

Reagents, Controls & Accessories

AB64201

Pepsin Solution (Antigen Retrieval)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Pepsin Solution (Antigen Retrieval) (AB64201)

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Reagents, Controls & Accessories

AB970

Trypsin Antigen Retrieval Solution

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Reagents, Controls & Accessories

AB64218

Hydrogen Peroxide Blocking Reagent

Immunohistochemistry (Frozen sections) - Hydrogen Peroxide Blocking Reagent (AB64218)

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Product promise

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