Proteinase K antigen retrieval solution is a ready-to-use, single-component solution designed for proteolytic-induced antigen retrieval (PIER) in immunohistochemistry (IHC) and pre-treatment for in situ hybridization (ISH).
- Cited in over 25 publications
- Ready-to-Use – no preparation needed for immediate application
- Incubate tissue section for 5-7 minutes at room temperature in IHC
- Incubate tissue section for 15 minutes at 37°C in ISH
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Publications
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- Arthritis research & therapy 26:22024Exosome-mediated delivery of super-repressor IκBα alleviates inflammation and joint damages in rheumatoid arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHae-In Lee,Min-Joo Ahn,Jae-Kwang Yoo,So-Hee Ahn,Seon Young Park,Hyangmi Seo,Moon-Ju Kim,Yu Jeong Lee,Hyun Hee Jang,Seung Cheol Shim,Eun Jeong Won,Cheolhyoung Park,Chulhee Choi,Tae-Jong KimPubMed 38167497
- Nature 619:801-8102023Spatially resolved multiomics of human cardiac niches.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKazumasa Kanemaru,James Cranley,Daniele Muraro,Antonio M A Miranda,Siew Yen Ho,Anna Wilbrey-Clark,Jan Patrick Pett,Krzysztof Polanski,Laura Richardson,Monika Litvinukova,Natsuhiko Kumasaka,Yue Qin,Zuzanna Jablonska,Claudia I Semprich,Lukas Mach,Monika Dabrowska,Nathan Richoz,Liam Bolt,Lira Mamanova,Rakeshlal Kapuge,Sam N Barnett,Shani Perera,Carlos Talavera-López,Ilaria Mulas,Krishnaa T Mahbubani,Liz Tuck,Lu Wang,Margaret M Huang,Martin Prete,Sophie Pritchard,John Dark,Kourosh Saeb-Parsy,Minal Patel,Menna R Clatworthy,Norbert Hübner,Rasheda A Chowdhury,Michela Noseda,Sarah A TeichmannPubMed 37438528
- Turkish neurosurgery 33:162-1702022The Matrix Metalloproteinase Inhibitor Batimastat Reduces Epidural Fibrosis After Laminectomy in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiya Yurtal,Huseyin Ozkan,Tuncer Kutlu,Mehmet Deveci,Boran Urfali,Senem UrfaliPubMed 36482856
- Respiratory research 23:2492022Effects of tocilizumab and dexamethasone on the downregulation of proinflammatory cytokines and upregulation of antioxidants in the lungs in oleic acid-induced ARDS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFunda Terzi,Beste Demirci,İrfan Çınar,Mohammad Alhilal,Huseyin Serkan ErolPubMed 36115998
- Bio-protocol 12:2022Immunohistochemistry of Immune Cells and Cells Bound to Administered Antibodies in Liver, Lung, Pancreas, and Colon of B6/lpr Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKieran Adam,Adam MorPubMed 35978576
- Journal of the American Heart Association 11:e0251812022Myeloid Poldip2 Contributes to the Development of Pulmonary Inflammation by Regulating Neutrophil Adhesion in a Murine Model of Acute Respiratory Distress Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiwei Ou,Elena Dolmatova,Rohan Mandavilli,Hongyan Qu,Georgette Gafford,Taylor White,Alejandra Valdivia,Bernard Lassègue,Marina S Hernandes,Kathy K GriendlingPubMed 35535614
- Scientific reports 12:14652022Aluminium co-localises with Biondi ring tangles in Parkinson's disease and epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew John Mold,Christopher ExleyPubMed 35087154
- Neuropathology : official journal of the Japanese Society of Neuropathology 42:66-732021An autopsy case of adult-onset neuronal intranuclear inclusion disease with perivascular preservation in cerebral white matter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaisuke Yoshii,Takashi Ayaki,Takafumi Wada,Akihiko Ozaki,Toru Yamamoto,Yoshimi Miyagi,Hideto Senzaki,Ryosuke TakahashiPubMed 34954850
- Frontiers in physiology 12:6734972021Distribution of Cardiac and Renal Corin and Proprotein Convertase Subtilisin/Kexin-6 in the Experimental Model of Cardio-Renal Syndrome of Various Severities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmad E Khoury,Ahmad Fokra,Safa Kinaneh,Yara Knaney,Doron Aronson,Zaid AbassiPubMed 34733169
- Journal of food biochemistry 45:e139652021Tyrosol retards induction of fibrosis in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTuncer Kutlu,Hüseyin Özkan,Mehmet GüvençPubMed 34636053
Key facts
- Applications
- IHC-P, ISH
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ISH | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Proteinase K antigen retrieval solution is a ready-to-use, single-component solution designed for proteolytic-induced antigen retrieval (PIER) in immunohistochemistry (IHC) and pre-treatment for in situ hybridization (ISH).
- Cited in over 25 publications
- Ready-to-Use – no preparation needed for immediate application
- Incubate tissue section for 5-7 minutes at room temperature in IHC
- Incubate tissue section for 15 minutes at 37°C in ISH
Key facts
- Applications
- IHC-P, ISH
- Form
- Liquid
- Storage buffer
pH: 7 - 7.4
Preservative: 0.1% Sodium azide
Constituents: Tris buffer, 10% Glycerol (glycerin, glycerine)
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
ab64220 is a single-component, ready-to-use proteinase K solution for use in protease-induced antigen retrieval during IHC (PIER) and pre-treatment for in situ hybridization.
Staining Protocol:
- IHC procedure: Incubate tissue section for 5 to 7 minutes at room temperature; Rinse and proceed with immunostaining.
- General ISH procedure: Incubate tissue section for 15 minutes at 37oC; Rinse and proceed with hybridization.
Other products for IHC
Other enzymes for antigen retrieval include: Pepsin Solution ab64201 and Trypsin Antigen Retrieval Kit ab259256.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteinase K Antigen Retrieval Solution (ab64220)
ab64220 Proteinase K used with aa anti-Pan CK antibody on Human skin
Downloads
Product protocols
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- Visit troubleshooting
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