Red Blood Cell (RBC) Lysis Buffer (1x)

Product details

This product is manufactured by BioVision, an Abcam company and was previously called 5830 1X Red Blood Cell Lysis Buffer. 5830-100 is the same size as the 100 ml size of ab286858.

Human whole blood is composed of 45% red blood cells. Without the removal of red blood cells, it is difficult to analyze the phenotype and function of leukocytes in whole blood. Hemoglobin and other red blood cell contents can also interfere with several chemical assays. This Red Blood Cell (RBC) Lysis Buffer (1x) (ab286858) uses ammonium chloride method to lyse red blood cells without affecting leukocytes, normal tissue, or tumor cells.

Protocol for using Red Blood Cell (RBC) Lysis Buffer:
1. Red Blood Cell lysis in whole blood: Add 1 volume of whole blood to 20 volumes of 1X Red Blood Cell (RBC) Lysis Buffer. Incubate for 5 -10 minutes at RT. Centrifuge at 400 x g for 5 min. and remove the supernatant carefully. Resuspend the cell pellet in appropriate buffer. Cells are ready for further analysis.
Notes:
a. Always take precautions when handling human samples.
b. Repeat the above lysis protocol as needed to remove all traces of RBC.
2. Red Blood Cell Lysis in tissue or tumor samples: After initial dissociation of tissue or tumor into single cells, centrifuge cells at 400 x g for 5 min. at RT. Remove the supernatant. For less than 1X10⁸ cells, resuspend the cell pellet in 5 ml 1X Red Blood Cell (RBC) Lysis Buffer. Incubate for 5-10 min. at RT. Centrifuge at 400 x g for 5 min. at RT and remove the supernatant carefully. Resuspend the cell pellet in appropriate buffer. Cells are ready for further analysis.
Note: For more than 1X10⁸ cells, resuspend the cell pellet in 10 ml of 1X Red Blood Cell (RBC) Lysis Buffer.