Rhodamine Phalloidin Reagent

Product details

Rhodamine Phalloidin Reagent ab235138 is one of a series of phalloidin conjugates that are used to stain actin filaments in fluoresence microscopy. The tetramethyl rhodamine dye can be easily detected with a fluorescent microscope at Ex/Em = 546/575 nm.

Each 300 test product includes 1 vial containing 30 µg of Rhodamine-conjugated-phalloidin in 30 µL. We recommend using this at a 1:1000 dilution, see protocol booklet for more instructions.

Phalloidin conjugates are convenient probes for labeling, identifying and quantitating animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.

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Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488 (ab176753), Phalloidin-iFluor 555 (ab176756), Phalloidin-iFluor 647 (ab176759), and Phalloidin-iFluor 594 (ab176757), search the website to see all phallodin conjugates, or read the phalloidin staining protocol.

Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.

When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.

Reagent Preparation

Before opening, briefly centrifuge the small vial at low speed to ensure all contents are collected at the bottom and to prevent loss of material.