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AB288006

RIPA Buffer

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(7 Publications)

RIPA Buffer (ab288006) is a ready-to-use 1X lysis buffer suitable for protein extraction from adherent and suspension cultured cells and tissues for radioimmunoprecipitation assays.

- Suitable buffer for your western blot (WB), SDS-PAGE, ELISA and IP workflow
- Useful for lysis of whole-cell extracts and membrane-bound proteins
- Available in different sizes to fit your experimental needs

Key facts

Form

Liquid

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Product details

A ready-to-use buffer useful for lysing cells and tissues for radioimmunoprecipitation assay. RIPA buffer also results in low background in immunoprecipitation/pull down assays.
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

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Target data

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell biochemistry and function 43:e70061 PubMed40011226

2025

Matrix Stiffness Regulates Interleukin-10 Secretion in Human Microglia (HMC3) via YAP-Mediated Mechanotransduction.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Fang,Haiying Jia,Shaoshan Pan,Qian Liu,Qian Wang,Ye Feng,Weiping Ding,Tianzhi Luo

Oncology letters 28:416 PubMed38988443

2024

Purine metabolite inosine induced by transforming growth factor‑β promotes epithelial‑mesenchymal transition in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weimin Hu,Ling Cheng,Jian Zhao,Yonghui Wu,Ying Lin,Jun Yin

Journal of translational medicine 22:5 PubMed38169393

2024

PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Tan,Qi Pan,Chao Yu,Xinyu Zhai,Jianyi Gu,Le Tao,Dongliang Xu

Antioxidants (Basel, Switzerland) 12: PubMed37891964

2023

Combination of Cysteine and Glutathione Prevents Ethanol-Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeongyeong Kim,Hyung Joo Suh,Ki-Bae Hong,Eun-Jin Jung,Yejin Ahn

Obesity (Silver Spring, Md.) 31:2689-2698 PubMed37840435

2023

Lower muscle protein synthesis in humans with obesity concurrent with lower expression of muscle IGF1 splice variants.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo D S Freitas,Katon A Kras,Lori R Roust,Elena A De Filippis,Scot R Kimball,Matthew Buras,Christos S Katsanos

Cell cycle (Georgetown, Tex.) 22:1637-1653 PubMed37345432

2023

p18INK4C and BRCA1 inhibit follicular cell proliferation and dedifferentiation in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Bai,Xiong Liu,Xu Zhang,Zhuo Mao,He Wen,Jinshan Ma,Xin-Hai Pei

Nature biotechnology 41:1582-1592 PubMed36823354

2023

Retrospective analysis of enhancer activity and transcriptome history.

Applications

Unspecified application

Species

Unspecified reactive species

Ruben Boers,Joachim Boers,Beatrice Tan,Marieke E van Leeuwen,Evelyne Wassenaar,Erlantz Gonzalez Sanchez,Esther Sleddens,Yasha Tenhagen,Eskeatnaf Mulugeta,Joop Laven,Menno Creyghton,Willy Baarends,Wilfred F J van IJcken,Joost Gribnau
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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